VPE, RVPE and UHMWPE with no antibiotics (control) were incubated in PBS for 12 months. PBS was replaced every week until the end of study. At 12 months, no vancomycin and rifampin could be detected by both UV-Vis and HPLC. Samples were then washed twice with PBS and blocked for 30 min using 10% fetal bovine serum in distilled, deionized water (blocking buffer). Samples were then incubated with mouse anti-VAN IgM (1:300; U.S. Biologicals, Swampscott, MA) in blocking buffer at 4°C for 12 hr, washed with PBS, and incubated with AlexaFluor 488-coupled goat anti-mouse IgM (A-21042, Thermo Fisher Scientific) diluted 1:300 in blocking buffer at room temperature for 1 hr. Samples were then washed three times in PBS, followed by 2×30 min incubations in PBS. Particles were then visualized using inverted fluorescence microscope (Nikon Eclipse TE2000).
Alexafluor 488 coupled goat anti mouse igm a 21042
Manufactured by Thermo Fisher Scientific
AlexaFluor 488-coupled goat anti-mouse IgM (A-21042) is a secondary antibody used for fluorescent detection of mouse IgM in immunoassays. The antibody is conjugated to the AlexaFluor 488 fluorescent dye.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexafluor 488 coupled goat anti mouse igm a 21042
1
Evaluation of Antibiotic Binding on Biomaterials
2
Evaluation of Antibiotic Binding on Biomaterials
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VPE, RVPE and UHMWPE with no antibiotics (control) were incubated in PBS for 12 months. PBS was replaced every week until the end of study. At 12 months, no vancomycin and rifampin could be detected by both UV-Vis and HPLC. Samples were then washed twice with PBS and blocked for 30 min using 10% fetal bovine serum in distilled, deionized water (blocking buffer). Samples were then incubated with mouse anti-VAN IgM (1:300; U.S. Biologicals, Swampscott, MA) in blocking buffer at 4°C for 12 hr, washed with PBS, and incubated with AlexaFluor 488-coupled goat anti-mouse IgM (A-21042, Thermo Fisher Scientific) diluted 1:300 in blocking buffer at room temperature for 1 hr. Samples were then washed three times in PBS, followed by 2×30 min incubations in PBS. Particles were then visualized using inverted fluorescence microscope (Nikon Eclipse TE2000).
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