The anti-mouse IgG conjugated with HRP was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). All the antibodies for the Western blot experiments were obtained from Cell Signaling Technology® (Danvers, MA, USA.). All the other reagents and chemicals were purchased from Merck® (Darmstadt, Germany). The cell culture wares, including plates and flasks (polystyrene coated), were obtained from Nunc Cell Culture® (Thermo Fisher Scientific, Waltham, MA, USA).
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reagent
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a colorimetric assay reagent used to measure cell metabolic activity. It is a yellow tetrazolium salt that is reduced to a purple formazan product by dehydrogenase enzymes in living cells.
Lab products found in correlation
2 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reagent
In Vitro Cell Cytotoxicity Evaluation
The anti-mouse IgG conjugated with HRP was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). All the antibodies for the Western blot experiments were obtained from Cell Signaling Technology® (Danvers, MA, USA.). All the other reagents and chemicals were purchased from Merck® (Darmstadt, Germany). The cell culture wares, including plates and flasks (polystyrene coated), were obtained from Nunc Cell Culture® (Thermo Fisher Scientific, Waltham, MA, USA).
Molecular Mechanisms of Oxidative Stress
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