Ber act35

Manufactured by BioLegend

Sourced in United States

The BER-ACT35 is a laboratory device designed for cell activation. It functions by stimulating and inducing the activation of cells through the interaction of the BER-ACT35 with cellular receptors.

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Lab products found in correlation

Cd138 percpcy5, by BioLegend (1 mentions) Cd40l bv605, by BioLegend (1 mentions) Ccr7 pecy7 3d12, by BD (1 mentions) Cd3 percp cy5, by BioLegend (1 mentions) Pd 1 pe, by BioLegend (1 mentions) Cd19 bv650, by BioLegend (1 mentions) Trucount beads, by BD (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Lsr 2, by BD (1 mentions)

Spelling variants of this product

OX40 (clone Ber-ACT35, (2 citations)

2 protocols using ber act35

Comprehensive Immune Cell Phenotyping

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Directly conjugated antibodies were purchased from BD Biosciences (San Jose, CA, USA): CCR7-PECy7 (3D12), CD3-PerCPCy5.5 and CD3-V500 (UCHT1), Foxp3-AF488 (259D/C7), CD25-AF700 (M-A251), CD4-APCH7 (RPA-T4), CXCR5-AF647 (RF8.B2), CD45RA-PECF594 (HI100), CD19-APCCy7 (SJ25C1), IgM-FITC (G20-127), IgD-PECF594 (IA6-2), CD38-V450 (HB7), CD138-PerCPCy5.5 (MI15), CD21-APC (B-LY4), CD27-PE (M-T271), IgG-BV605 (G18-145) or BioLegend (San Diego, CA, USA): PD1-PE (EH12.2H7), CD19-BV650 (HIB19), CD57-PB (HCD57), ICOS-FITC and ICOS-PerCPCy5.5 (C398.4A), OX40-PECy7 (BER-ACT35), CD40L-BV605 (24–31).

Colineau L., Rouers A., Yamamoto T., Xu Y., Urrutia A., Pham H.P., Cardinaud S., Samri A., Dorgham K., Coulon P.G., Cheynier R., Hosmalin A., Oksenhendler E., Six A., Kelleher A.D., Zaunders J., Koup R.A., Autran B., Moris A, & Graff-Dubois S. (2015). HIV-Infected Spleens Present Altered Follicular Helper T Cell (Tfh) Subsets and Skewed B Cell Maturation. PLoS ONE, 10(10), e0140978.

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Quantifying Immune Cell Populations

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Bovine serum albumin (BSA) and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO). OX40 was detected using APC-conjugated anti-human OX40 clone Ber-ACT35 (Biolegend). Flow cytometry data was collected by a BD LSRII flow cytometer (Becton Dickinson, Franklin Lakes, NJ) and flow cytometry standard (fcs) data analyzed using Flowjo software (Flowjo LLC, Ashland, OR). Absolute CD4 and CD8 cell counts for the rhesus macaque study were generated using TruCount Beads (Becton Dickinson) and is described further in Supplementary Methods.

Oberst M.D., Augé C., Morris C., Kentner S., Mulgrew K., McGlinchey K., Hair J., Hanabuchi S., Du Q., Damschroder M., Feng H., Eck S., Buss N., de Haan L., Pierce A.J., Park H., Sylwester A., Axthelm M.K., Picker L., Morris N.P., Weinberg A, & Hammond S.A. (2018). Potent Immune Modulation by MEDI6383, an Engineered Human OX40 Ligand IgG4P Fc Fusion Protein. Molecular cancer therapeutics, 17(5), 1024-1038.

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