Spleen single-cell suspensions were collected from the mice, filtered with a cell strainer, distributed into 2 sets and were suspended in 50 μL of cold PBS containing 2% fetal calf serum. The first set of cells was stained with V450-conjugated anti-CD45 (BD Bioscience), BB515-conjugated anti-major histocompatibility complex II (MHCII) (BD Bioscience), phycoerythrin-conjugated anti-CD11c (BD Bioscience) for the detection of activated DCs, anti-CD11b+/F4/80+ for macrophages, and anti-CD11b+/Ly6G+ for neutrophils. The other set of cells was stained with peridinin chlorophyll protein complex-conjugated anti-CD3 (BD Bioscience), fluorescein isothiocyanate-conjugated anti-CD4 (eBiosience, San Diego, CA, USA), APC-conjugated anti-CD8, and phycoerythrin-conjugated anti-Foxp3 for the detection of T cells. The cells were analyzed by flow cytometry (LSR II; BD Bioscience). Flow cytometry was performed using an BD FACSCantoIIand analyzed by FlowJo software.
Peridinin chlorophyll protein complex conjugated anti cd3
Manufactured by BD
Sourced in United States
Peridinin chlorophyll protein complex-conjugated anti-CD3 is a laboratory reagent. It consists of an anti-CD3 antibody conjugated to a peridinin chlorophyll protein complex. This complex serves as a fluorescent label.
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Lab products found in correlation
V450 conjugated anti cd45, by BD (2 mentions)
Bb515 conjugated anti major histocompatibility complex 2 mhcii, by BD (2 mentions)
Phycoerythrin conjugated anti cd11c, by BD (2 mentions)
Allophycocyanin conjugated anti cd4, by BD (1 mentions)
Fitc conjugated anti hla dr, by BD (1 mentions)
Pe conjugated anti cd38, by BD (1 mentions)
3 protocols using peridinin chlorophyll protein complex conjugated anti cd3
1
Multiparametric Analysis of Murine Splenocytes
2
Quantifying T-cell Activation Using Flow Cytometry
3
Identification of Activated Dendritic Cells and Regulatory T Cells
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For the analysis, harvested cells from each mouse were distributed into 2 sets and were suspended in 50 μL of cold PBS containing 2% fetal calf serum. The first set of cells was stained with V450-conjugated anti-CD45 (BD Bioscience), BB515-conjugated anti-major histocompatibility complex II (MHCII) (BD Bioscience), phycoerythrin-conjugated anti-CD11c (BD Bioscience) and anaphase-promoting complex (APC)-conjugated anti-CD86 (BD Bioscience) for the detection of activated DCs. The other set of cells was stained with peridinin chlorophyll protein complex-conjugated anti-CD3 (BD Bioscience), fluorescein isothiocyanate-conjugated anti-CD4 (eBiosience, San Diego, CA, USA), APC-conjugated anti-CD25, and phycoerythrin-conjugated anti-Foxp3 for the detection of regulatory T cells. The cells were analyzed by flow cytometry (LSR II; BD Bioscience). The CD11c+MHCIIhigh cells were considered as migratory DCs (Supplementary Fig. S1 ).9 (link)
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