ELISA was done to measure the concentration of SDF-1α protein in conditioned media of NC65, NC66, and HEK293. The culture supernatant was harvested and centrifuged to remove cellular debris, and Human CXCL12 ELISA kit (R&D Systems, UK) was used to measure chemokine production in each supernatant according to the manufacturer's instructions. The ELISA assay was carried out twice in duplicate.
Human cxcl12 elisa kit
Manufactured by R&D Systems
Sourced in United Kingdom
The Human CXCL12 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure CXCL12 levels in human cell culture supernatants, serum, and plasma samples. The kit utilizes a specific antibody coated on a microplate to capture CXCL12 from the samples, which is then detected using a second CXCL12-specific antibody conjugated to horseradish peroxidase. The assay provides a linear range for CXCL12 quantification.
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Lab products found in correlation
3 protocols using human cxcl12 elisa kit
1
ELISA-based Quantification of SDF-1α
2
Quantitative CXCL12 Secretion Assay
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Supernatants from each group were collected, and the concentration of CXCL12 was detected using a human CXCL12 ELISA kit (R&D Systems), and the CXCL12 level in the supernatant was measured using a Quantikine kit within 30 minutes according to the manufacturer’s instructions (R&D Systems). High intra-assay and inter-assay precision was established by the manufacturer. The absorbance was recorded using a microplate reader (ELx808, Bio-Tek Instruments), and the data collection and analysis were performed using Gen5 software Version 1.08 (Bio-Tek Instruments).
3
BRAFV600E Impacts Thyrocyte CXCL12 Secretion
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In total, 5 × 105 cells of control, BRAFV600E or BRAFV600E/shCXCL12 lentivirus infected thyrocyte were seeded and maintained in 2 ml of F12K media. After 48 h, the media was harvested and analysed secreted CXCL12 protein using human CXCL12 ELISA kit (R&D System).
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