Pcmv6 ddk myc tmem17

Manufactured by OriGene

Sourced in United States

PCMV6-ddk-myc-TMEM17 is a plasmid vector that contains the human TMEM17 gene with a DDK and Myc tag. The plasmid is designed for mammalian expression and can be used for the overexpression of the TMEM17 protein in cell lines.

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Lab products found in correlation

Pcmv6 ddk myc, by OriGene (2 mentions) Tmem17 sirna sc 94962, by Santa Cruz Biotechnology (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Nc sirna, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Nc sirna sc 37007, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

PCMV6-ddk-myc (21 citations)

2 protocols using pcmv6 ddk myc tmem17

Plasmid Transfection Protocol

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Plasmids pCMV6-ddk-myc and pCMV6-ddk-myc-TMEM17 were purchased from Origene (Rockville, MD, USA). TMEM17-siRNA (sc-94962) and NC-siRNA (sc-37007) was purchased from Santa Cruz Biotechnology. Transfection was carried out using the Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions.

Zhang X., Zhang Y., Miao Y., Zhou H., Jiang G, & Wang E. (2017). TMEM17 depresses invasion and metastasis in lung cancer cells via ERK signaling pathway. Oncotarget, 8(41), 70685-70694.

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Overexpression of TMEM17 in Cells

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Plasmids pCMV6-ddk-myc and pCMV6-ddk-myc-TMEM17 were purchased from OriGene (Rockville, MD, USA). TMEM17-siRNA (sc-94962) and NC-siRNA (sc-37007) were purchased from Santa Cruz Biotechnology Inc. Transfection was performed using Lipofectamine 3000 reagent (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions.

Zhao Y., Song K., Zhang Y., Xu H., Zhang X., Wang L., Fan C., Jiang G, & Wang E. (2018). TMEM17 promotes malignant progression of breast cancer via AKT/GSK3β signaling. Cancer Management and Research, 10, 2419-2428.

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