For aPTT tests, Dade Actin FSL and Dade Actin FS aPTT reagents (Siemens Healthineers) were used for aPTT screening and confirmatory tests, respectively. LA1 Screening and LA2 Confirmation reagents (Siemens Healthineers) were employed for dRVVT screening and confirmatory tests, respectively. Additional coagulation indices measured prior to LA testing, including prothrombin time, thrombin time, and fibrinogen level (Clauss method), were measured by the same analyzer using Thromborel S, Thromboclotin, and Dade Innovin thrombin reagents (Siemens Healthineers), respectively. In a 1:1 mixing test, a commercial standard human plasma (Siemens Healthineers) was used as the normal pooled plasma (NPP). All assays were performed on a Sysmex CS−2500 Coagulation analyzer (Sysmex).
Dade actin fsl
Manufactured by Siemens
Sourced in Germany
The Dade Actin FSL is a lab equipment product designed for use in clinical laboratories. It serves as a coagulation reagent that aids in the determination of the activated partial thromboplastin time (aPTT) in human plasma samples.
Automatically generated - may contain errors
Lab products found in correlation
Biophen heparin lrt, by Hyphen Biomed (3 mentions)
Dade innovin, by Siemens (3 mentions)
Cs2100i, by Sysmex (3 mentions)
Dade thrombin reagent, by Siemens (2 mentions)
Cobas 8000, by Roche (2 mentions)
Innovance, by Siemens (2 mentions)
Innovance d dimer, by Siemens (2 mentions)
Thromboclotin, by Siemens (1 mentions)
Cs 2500 coagulation analyzer, by Sysmex (1 mentions)
Thromborel s, by Siemens (1 mentions)
Elecsys ferritin, by Roche (1 mentions)
Xn 9000 analyzer, by Sysmex (1 mentions)
Dade fibrinogen determination reagents, by Siemens (1 mentions)
Innovin, by Siemens (1 mentions)
6 protocols using dade actin fsl
1
Coagulation Assays Standardization Protocol
2
Comprehensive Hemostasis Panel Evaluation
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The aPTT (Dade Actin FSL; Siemens, Marburg, Germany), PT (Dade Innovin; Siemens), fibrinogen level (Clauss method, Dade Thrombin Reagent; Siemens), FVIII activity (Dade Actine FS and FVIII deficient plasma; Siemens), D-dimer (INNOVANCE D-dimer; Siemens), antithrombin (INNOVANCE; Siemens), and anti-Xa (Biophen Heparin LRT; Hyphen Biomed, Neuville-Sur-Oise, France) were measured on a Sysmex CS2100i (Sysmex Corporation, Kobe, Hyogo, Japan) hemostasis analyzer. Samples for the anti-Xa test were first diluted 2x with pooled reference plasma containing ∼100% ATIII and the anti-Xa activity was subsequently determined using specific calibration lines for UFH (aXa-UFH) (Biophen UFH Calibrator; Hyphen Biomed) or low-molecular-weight heparin (LMWH) (aXa-LMWH) (Biophen Heparine Calibrator; Hyphen Biomed). The aPTT (Cephascreen; Stago, Paris, France) was also performed on a STA-R Max2 analyzer (Stago). Thrombocyte count was determined using a Sysmex XN-9000 analyzer (Sysmex). C-reactive protein (CRP, third generation, Roche Diagnostics, Basel, Switzerland) and ferritin (Elecsys ferritin, Roche) were performed on the COBAS8000 by Roche Diagnostics.
3
Coagulation Profile Assessment of Venous Blood Samples
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Each 4.5 mL venous blood sample in a 5.0 mL BD blood collection tube with 0.5 mL 3.2% sodium citrate (Catalog No. 366415, Becton Dickinson, Franklin Lakes, NJ, USA) was centrifuged at 1,500 g for 10 minutes (KUBOTA 4000 centrifuge, Fujioka Japan). Using the SYSMEX CA-1500 Coagulation Analyzer (Kobe, Japan), the plasma obtained was quantified for six coagulation parameters including prothrombin time (PT) (Dade INNOVIN, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany), activated partial thromboplastin time (APTT) (Dade Actin FSL, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany), and fibrinogen (Dade Fibrinogen Determination Reagents, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany) using the clotting method as well as D-dimer with the immuno-turbidity approach (INNOVANCE D-dimer, Siemens Healthcare Diagnostics Products, GmbH. 35041 Marburg/Germany), antithrombin III (Siemens Antithrombin III assay, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany) and protein C (Dade Behring Protein C Reagent, Siemens Healthcare Diagnostics Products, GmbH. 35041, Marburg/Germany) using the chromogenic method according to the manufacturer's instructions.
4
aPTT and anti-Xa Assay in COVID-19
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The aPTT (Dade Actin FSL; Siemens, Marburg, Germany) and anti-Xa (Biophen Heparin LRT; Hyphen Biomed, Neuville-Sur-Oise, France) were performed on a Sysmex CS2100i (Sysmex Corporation, Kobe, Hyogo, Japan) hemostasis analyzer in 3.2% citrated blood. Local aPTT reference values were 23–32s. For the anti-Xa measurement, samples of COVID-19 patients (18 μl) were diluted three times with reference pooled plasma (36 μl). Anti-Xa activity was determined using an LMWH calibration line (anti-Xa-LMWH; Hyphen Biomed).
5
Comprehensive Blood Analysis in COVID-19 Patients
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Complete blood count (leukocyte count, platelet count, hemoglobin, and hematocrit) was performed on a Sysmex XN-9000 analyzer (Sysmex Corporation, Kobe, Hyogo, Japan) using EDTA blood. PT (Dade Innovin; Siemens, Marburg, Germany), aPTT (Dade Actin FSL; Siemens), fibrinogen concentration (Clauss method, Dade Thrombin Reagent; Siemens), D-dimer concentration (Innovance; Siemens), and Anti-Xa activity (Biophen Heparin LRT; Hyphen Biomed, Neuville-Sur-Oise, France) were measured on a Sysmex CS2100i (Sysmex Corporation) in 3.2% citrated blood. For the anti-Xa measurement, COVID-19 patient samples (18 μl) were three times diluted with reference pooled plasma (36 μl). Anti-Xa activity was determined using an LMWH calibration line (aXa-LMWH; Hyphen Biomed). UFH activity was subsequently calculated with a previously determined formula: UFH anti-Xa = 1.55 * LMWH anti-Xa (26 (link)). Bilirubin (Bilirubin Total, third-generation; Roche Diagnostics, Basel, Switzerland) and creatinine (Enzymatic Reagent; Roche Diagnostics) were determined in serum on a COBAS® 8000 (Roche Diagnostics).
6
Coagulation Analysis Using Automated Sysmex
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CWA was performed with Sysmex CN-6000 automated coagulation analyser (Sysmex Corporation, Kobe, Japan) with Dade Actin FSL (Siemens Healthcare, Marburg, Germany) for aPTT CWA and Innovin (Siemens Healthcare, Marburg, Germany) for PT CWA, as per International Society of Haemostasis and Thrombosis (ISTH) Scientific and Standardization Committee recommendation [15 (link)]. Four quantitative parameters were recorded- “Min1” (maximum velocity), “Min2” (maximum acceleration), “Max2” (maximum deceleration), and “Delta change” (difference between initial maximum and final maximum values of light transmittance).
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