Pha 543613 hydrochloride

Scopolamine hydrobromide (Tocris), PHA-543613 hydrochloride (Tocris), and memantine hydrochloride (Tocris) were dissolved in physiological saline to create a final injection volume of 1 ml/kg. Scopolamine was injected intraperitoneally (i.p.) 10 min before experimental sessions, and PHA-543613 and memantine were injected subcutaneously (s.c.) 40 min before experiments (30 min before scopolamine administration). In treatments, where scopolamine, memantine and/or PHA-543613 were not administered, compounds were replaced with saline (vehicle, VEH) injected through the corresponding route of administration. In co-administration treatments, memantine and PHA-543613 were administered consecutively in two separate subcutaneous injections performed on the opposite sides of the body.

PHA 543613 hydrochloride (Tocris Bioscience, Bristol, UK; 17, 18) was dissolved in sterile water and intra-peritoneally injected at the concentration of 6 mg/kg (300 μL/300 g body weight) 2 h before 6-OHDA lesioning and then at days 2, 4, and 6 post-lesion (cumulative dose of 24 mg/kg). Twelve rats were included in this study and separated into two groups as follows: seven lesioned rats received the treatment (PHA group) and five lesioned rats received intra-peritoneally injection of vehicle at the same time points (Sham group).