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The Lidk06801 is a laboratory equipment product used for genetic research. It is a tool designed to facilitate the handling and manipulation of Drosophila, a model organism commonly used in genetic studies. The core function of the Lidk06801 is to provide a controlled environment for the culture and observation of Drosophila specimens.

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2 protocols using lidk06801

1

Drosophila Genetic Background Preparation

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All flies were maintained on a standard cornmeal/molasses diet at 25 °C and 75% humidity on a 12-hour day and 12-hour night cycle. The following lines were acquired from the Bloomington Stock Center: lid10424 (BL# 12367) and lidk06801 (BL# 10403). PSRFM1 was provided by Kristin White (Massachusetts General Hospital, Charlestown, MA). The generation and verification of the knockout lines and tagged transgenes is described in Shalaby et al.32 (link). Flies were outcrossed in groups of >20 for at least 5 generations to the w* Berlin genetic background for behavioral analyses.
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2

Drosophila Genetic Crosses for Functional Analysis

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The following lines were acquired from the Bloomington Stock Center: w1118 (BL# 38690), hsFlp,hsIscel/CyO (BL # 6934), nanos-Gal4 (BL# 32179), Tubulin-Gal4 (BL# 5138), eyeless-Gal4 (BL# 8227), In(1)whitem4 (BL# 807), Sb[V] (BL# 878), Cre (BL# 1501) lid10424 (BL# 12367), lidk06801 (BL# 10403). PSRFM1 was provided by Kristin White (Massachusetts General Hospital, Charlestown, MA), FRT40A, UTX1 (link) was provided by Andreas Bergmann (M. D. Anderson Cancer Center, Houston, TX), FRT40A, UTXΔ was provided by Jürg Müller (MPI of Biochemistry, Chromatin and Chromosome Biology, Martinsried, Germany).
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