2-bromoethylamine hydrobromide, TRIS hydrochloride, trypsin, α-cyano-4-hydroxycinnamic acid (CHCA), acetylated cysteine (Ac-C), and acetic anhydride were purchased from Sigma (St. Louis, MO, USA). Glu-C was from Promega (Madison, WI, USA). Potassium phosphate was acquired from JT Baker (Phillipsburg, NJ, USA) and ammonium bicarbonate was from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). Concentrated sodium hydroxide was obtained from VWR Analytical (Radnor, PA, USA) and dithiothreitol was purchased from Bio-Rad (Hercules, CA, USA). Genentech (South San Francisco, CA, USA) provided the antibody, rituximab. Fmoc-Arg(Ptf) Wang resin and Fmoc-Cys(Trt)-OH were from Midwest Biotech (Fishers, IN, USA).
2 bromoethylamine hydrobromide
Manufactured by Merck Group
Sourced in United States
2-bromoethylamine hydrobromide is a chemical compound commonly used as a laboratory reagent. It is a colorless crystalline solid that is soluble in water and alcohol. The compound is often utilized in organic synthesis reactions and is a versatile building block in the preparation of various derivatives.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
1 methylimidazole, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dithiothreitol (dtt), by Bio-Rad (1 mentions)
Rituximab, by Genentech (1 mentions)
Tris hydrochloride, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid chca, by Merck Group (1 mentions)
Glu c, by Promega (1 mentions)
Trypsin, by Merck Group (1 mentions)
Acetic anhydride, by Merck Group (1 mentions)
Potassium phosphate, by Avantor (1 mentions)
Ammonium bicarbonate, by Mallinckrodt (1 mentions)
N n diisopropylethylamine, by Merck Group (1 mentions)
1 2 dioleoyl 3 trimethylammonium propane dotap, by Avanti Polar Lipids (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Sodium phosphate monobasic monohydrate, by Thermo Fisher Scientific (1 mentions)
Anhydrous sodium sulfate, by Thermo Fisher Scientific (1 mentions)
Sodium phosphate dibasic heptahydrate, by Thermo Fisher Scientific (1 mentions)
Anhydrous pyridine, by Merck Group (1 mentions)
9 protocols using 2 bromoethylamine hydrobromide
1
Antibody Characterization and Modification
2
Synthesis of Imidazole-based Compounds
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All the reagents used were of analytical grade and used without further purification. orthovaniline, Aniline, 2 chloroaninline, 4-chloroaniline, and 4-fluoro aniline were procured from Sd fine chemical company, India.1-methylimidazole, KPF6 and 2-bromoethylaminehydrobromide were procured from sigma Aldrich and used without further purification. All the solvents used were of spectroscopic grade.
3
Lipid-Polymer Hybrid Nanoparticle Synthesis
4
Synthesis and Characterization of Ionic Liquids
5
Peptide Synthesis Reagents and Solvents
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Triethylamine, triisopropylsilane
(TIS), 2-bromoethylamine
hydrobromide, Chloroform-d1, sodium borohydride,
dimethyl sulfoxide-d6, bromoacetic acid, N,N′-diisopropylcarbodiimide (DIC), 4-bromobenzyl
bromide, methanol-d4, and H-Rink Amide
ChemMatrix were purchased from Sigma-Aldrich. Ditert-butyl dicarbonate and (benzotriazol-1-yloxy)tripyrrolidinophosphonium
hexafluorophosphate (PyBOP) were purchased from Novabiochem. Solvents
for peptide synthesis (analytical grade) were purchased from Sigma-Aldrich
(dimethylformamide) and J. T. Baker (diethyl ether). Trifluoroacetic
acid (TFA) and N,N-diisopropylethylamine
(DIEA) were purchased from Iris Biotech. Ethyl acetate and acetic
acid were purchased from J. T. Baker. Chloroform and dimethyl sulfoxide
were purchased from Chempur. Se powder and ethyl alcohol were purchased
from POCH. Amino acids were purchased from PeptideWeb. 4-Methoxybenzyl
chloride was purchased from TCI. Solvents for LC-MS and HPLC measurements
were as follows: acetonitrile (MeCN), formic acid (HCOOH), and water
were purchased from chemsolve and J. T. Baker. The solvent (HPLC grade)
for the irradiation experiments and UV–vis measurement was
methanol (MeOH), which was purchased from J. T. Baker. The solvent
for GC-MS measurement was dichloromethane (DCM), which was purchased
from Chemsolve.
(TIS), 2-bromoethylamine
hydrobromide, Chloroform-d1, sodium borohydride,
dimethyl sulfoxide-d6, bromoacetic acid, N,N′-diisopropylcarbodiimide (DIC), 4-bromobenzyl
bromide, methanol-d4, and H-Rink Amide
ChemMatrix were purchased from Sigma-Aldrich. Ditert-butyl dicarbonate and (benzotriazol-1-yloxy)tripyrrolidinophosphonium
hexafluorophosphate (PyBOP) were purchased from Novabiochem. Solvents
for peptide synthesis (analytical grade) were purchased from Sigma-Aldrich
(dimethylformamide) and J. T. Baker (diethyl ether). Trifluoroacetic
acid (TFA) and N,N-diisopropylethylamine
(DIEA) were purchased from Iris Biotech. Ethyl acetate and acetic
acid were purchased from J. T. Baker. Chloroform and dimethyl sulfoxide
were purchased from Chempur. Se powder and ethyl alcohol were purchased
from POCH. Amino acids were purchased from PeptideWeb. 4-Methoxybenzyl
chloride was purchased from TCI. Solvents for LC-MS and HPLC measurements
were as follows: acetonitrile (MeCN), formic acid (HCOOH), and water
were purchased from chemsolve and J. T. Baker. The solvent (HPLC grade)
for the irradiation experiments and UV–vis measurement was
methanol (MeOH), which was purchased from J. T. Baker. The solvent
for GC-MS measurement was dichloromethane (DCM), which was purchased
from Chemsolve.
6
Polymer-Based Biomaterial Synthesis
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Poly (methyl vinyl ether-co-maleic anhydride) or poly (anhydride) (Gantrez® AN119) was supplied by Ashland (Ashland, OR, USA). Mannosamine hydrochloride, zein, mannitol, lysine, tween 20, 2-bromoethylamine-hydrobromide, trifluoroacetic acid and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (Madrid, Spain). Sucrose was supplied by Fagron (Barcelona, Spain). Ethanol formaldehyde, sodium hydroxide and dimethyl sulfoxide (DMSO) were supplied by Panreac (Barcelona, Spain). Acetone was obtained from VWR-Prolabo was provided by Invitrogen (Carlsbad, CA, USA). Tryptic soy broth (TSB) was obtained from bioMérieux (Marcv L’Etoile, France). RPMI 1640 and fetal bovine serum were obtained from Gibco-BRL (Thistle Scientific, Glasgow, UK). Coomassie brilliant blue and sample buffer were purchased from Bio Rad (Madrid, Spain).
7
Ferulic Acid-Crosslinked Gelatin Hydrogels
8
Inactivation of Rabies Virus for Immunization
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Rabies virus used for immunization of mice was inactivated with 2-bromoethylamine
hydrobromide (Sigma-Aldrich) as previously described [16 (link)]. The compound was dissolved in 0.5 M sodium hydroxide (NaOH) at the
concentration of 0.6 M and was converted to binary ethylenimine (BEI) by incubation at
37°C for 1 hr. The BEI was then added to the virus stock at a final concentration of 0.03
M, incubated for 18 hr at 37°C, and neutralized by adding sodium thiosulfate
(Sigma-Aldrich) to a final concentration of 2%. The inactivated virus was then mixed
thoroughly with aluminum hydroxide adjuvant (60% virus: 40% adjuvant).
hydrobromide (Sigma-Aldrich) as previously described [16 (link)]. The compound was dissolved in 0.5 M sodium hydroxide (NaOH) at the
concentration of 0.6 M and was converted to binary ethylenimine (BEI) by incubation at
37°C for 1 hr. The BEI was then added to the virus stock at a final concentration of 0.03
M, incubated for 18 hr at 37°C, and neutralized by adding sodium thiosulfate
(Sigma-Aldrich) to a final concentration of 2%. The inactivated virus was then mixed
thoroughly with aluminum hydroxide adjuvant (60% virus: 40% adjuvant).
9
Electrochemical Sensing of Biomolecules
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GO was supplied by Nanjing XFNano Materials Technology Company (Nanjing, China, https://www.xfnano.com). All the other chemicals such as 2-bromoethylamine hydrobromide, 1methylimidazole, MnCl 2 •4H 2 O, tetramethyl-ammonium, K 3 Fe(CN) 6 , K 4 Fe(CN) 6 , ascorbic acid (AA), uric acid (UA), glucose, guanine, adenine, dopamine hydrochloride, citric acid, sodium citrate, Bull Serum Albumin (BSA), Britton-Robinson (BR) buffer and phosphate buffered solution were obtained from Sigma (Shanghai, China, https://www.sigmaaldrich.com/ china-mainland.html). Fetal bovine serum (FBS, #10439024) was purchased from Thermo Fisher Scientific (https://www. thermofisher.com/cn/zh/home.html). All reagents were analytical grade and used according to the instructions. Ultrapure water (18.25 MΩ cm) was used for all experiments.
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