Thymus cell suspensions were prepared and stained as described (Hager-Theodorides et al., 2005 (link)) using combinations of the following directly conjugated antibodies at concentration of 1:100: (from BD Pharmingen) anti-γδPE (catalogue no. 553178); from eBioscience: anti-TCRβFITC (catalogue no. 11-5961-85), antiCD3PE (catalogue no. 12-0031-82), anti-CD24PE (catalogue no. 12-0241-82) and anti-CD69FITC (catalogue no. 11-0691-85); (from Biolegend) anti-CD3FITC (catalogue no. 100204), anti-CD5FITC (catalogue no. 100605), anti-Qa2FITC (catalogue no. 121709), anti-CD4APC (catalogue no. 116014), anti-CD5PE (catalogue no. 100607), anti-CD8PerCP/Cy5.5 (catalogue no. 100734), anti-CD4PerCP/Cy5.5 (catalogue no. 100539) and anti-CD8APC (catalogue no. 100712). Data were acquired on a C6 Accuri flow cytometer (BD Biosciences) and analysed using FlowJo software. Live cells were gated by FSC and SSC profiles. Data represent at least three experiments.
Anti tcrβfitc
Manufactured by Thermo Fisher Scientific
Anti-TCRβFITC is a fluorochrome-conjugated antibody that binds to the T cell receptor beta chain (TCRβ) on the surface of T cells. It is used for the identification and quantification of T cells in flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 fitc, by BioLegend (1 mentions)
Anti cd4 apc, by BioLegend (1 mentions)
Anti cd3 pe, by BioLegend (1 mentions)
Anti cd24 pe, by BioLegend (1 mentions)
Anti cd8 apc, by BioLegend (1 mentions)
Anti cd4 percp cy5, by BioLegend (1 mentions)
Anti cd8 percp cy5, by BioLegend (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
Anti cd69 fitc, by BioLegend (1 mentions)
Anti cd5fitc, by BioLegend (1 mentions)
Anti cd5pe, by BioLegend (1 mentions)
Anti cd4 apc efluor 780, by Thermo Fisher Scientific (1 mentions)
Anti cd4 pe, by Thermo Fisher Scientific (1 mentions)
Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions)
Anti cd11c pe, by BioLegend (1 mentions)
Anti cd11b alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Anti gl7 fitc, by BD (1 mentions)
Anti cd8 pe cy7, by Thermo Fisher Scientific (1 mentions)
Anti cd244, by Thermo Fisher Scientific (1 mentions)
Anti cd45r pe, by BD (1 mentions)
2 protocols using anti tcrβfitc
1
Thymus Cell Suspension Preparation and Staining
2
Cd244 Knockout Mice for Immunological Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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C57BL/6 (WT) mice were purchased from The Jackson Laboratories (Bar Harbor, ME).
Cd244-/- mice on the C57BL/6 background [12 (link)], were kindly provided by Dr. Raymond Welsh (University of Massachusetts Medical Center, Worcester, MA). Mice were maintained in the Translational Biomedical Research Center of the Medical College of Wisconsin and both genders were used for experiments in an age (6–12 week) and gender-matched manner. anti-CD45R-PE-Texas Red, anti-CD45R-PE, anti-GL7-FITC and anti-CD5-APC were purchased from BD Biosciences (San Jose, CA). CD21-eFluor 450, anti-CD23-PE-Cy7, anti-CD93-biotin, anti-CD4-APC-eFluor 780, anti-CD8-PE-Cy7, anti-TCRβ-FITC, anti-TCRβ-PE, anti-CD11b-Alexa Fluor 488, anti-CD244, anti-Foxp3-PE, anti-CD19-Alexa 700, anti-CD4-PE, anti-IgG-FITC and Streptavidin PE-Cy5.5 were purchased from eBioscience (San Diego, CA). Anti-CD11b-Brilliant Violet 605, anti-CD11c-PE, anti-NK1.1-APC, anti-IgM-APC Cy7, anti-IgD-Pacific Blue, anti-CD38-Alexa Fluor 647, anti-CD138-APC and rat anti-mouse IgG2a-biotin were purchased from Biolegend (San Diego, CA). Anti-IgM-FITC and the SBA Clonotyping System-B6/C57J-HRP were purchased from Southern Biotech (Birmingham, AL). 4-Hydroxy-3-nitrophenylacetyl (NP)-ficoll, NP-Chicken Gamma Globulin (CGG), NP(24)-PE and NP-BSA were purchased from Biosearch Technologies (Novato, CA).
Cd244-/- mice on the C57BL/6 background [12 (link)], were kindly provided by Dr. Raymond Welsh (University of Massachusetts Medical Center, Worcester, MA). Mice were maintained in the Translational Biomedical Research Center of the Medical College of Wisconsin and both genders were used for experiments in an age (6–12 week) and gender-matched manner. anti-CD45R-PE-Texas Red, anti-CD45R-PE, anti-GL7-FITC and anti-CD5-APC were purchased from BD Biosciences (San Jose, CA). CD21-eFluor 450, anti-CD23-PE-Cy7, anti-CD93-biotin, anti-CD4-APC-eFluor 780, anti-CD8-PE-Cy7, anti-TCRβ-FITC, anti-TCRβ-PE, anti-CD11b-Alexa Fluor 488, anti-CD244, anti-Foxp3-PE, anti-CD19-Alexa 700, anti-CD4-PE, anti-IgG-FITC and Streptavidin PE-Cy5.5 were purchased from eBioscience (San Diego, CA). Anti-CD11b-Brilliant Violet 605, anti-CD11c-PE, anti-NK1.1-APC, anti-IgM-APC Cy7, anti-IgD-Pacific Blue, anti-CD38-Alexa Fluor 647, anti-CD138-APC and rat anti-mouse IgG2a-biotin were purchased from Biolegend (San Diego, CA). Anti-IgM-FITC and the SBA Clonotyping System-B6/C57J-HRP were purchased from Southern Biotech (Birmingham, AL). 4-Hydroxy-3-nitrophenylacetyl (NP)-ficoll, NP-Chicken Gamma Globulin (CGG), NP(24)-PE and NP-BSA were purchased from Biosearch Technologies (Novato, CA).
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