Veritas plate reading luminometer

The activities of caspase-3, -8 and -9 in BMDMs subjected to the indicated treatments were detected using caspase-Glo 3/7, caspase-Glo 8 and caspase-Glo assay kits (cat. nos. G8090, G8200 and G8210, respectively; Promega Corporation) according to the manufacturer's protocols. BMDMs were seeded at a density of 1x10³ cells in 96-well plates and allowed to adhere overnight. Following incubation with the indicated drugs, caspase-Glo reagent was added to the cells at a 1:1 ratio. The contents were mixed and the cells were incubated for 1 h at room temperature. The luminescence signal of each sample was then detected using a Veritas plate-reading luminometer (Turner BioSystems) according to the manufacturer's instructions (25 (link)). The relative percentage of luminescence intensity was calculated by comparison to the vehicle control (26 (link)).

Cells were seeded at a density of 10 000 cells/well (100 μL DMEM containing 10% FBS) in 96‐well plates 24 h before transfection. Then 100 μL caspase‐Glo 3/7 reagent (G8092; Promega, Madison, WI 53711, USA) were added to each well 48 h after transfection. The cells were lysed at room temperature for 1 h. Luminescence was measured using a Veritas plate‐reading luminometer (Turner Biosystems, Sunnyvale, CA, California, USA).