Realtime glo mt luminescent kit

Manufactured by Promega

Sourced in United States

The RealTime-Glo MT Cell Viability Assay is a luminescent kit that provides a real-time measurement of cell viability in cell culture. The kit utilizes a proprietary NanoLuc luciferase technology to detect the reducing potential of viable cells.

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Lab products found in correlation

Cell death detection elisa kit, by Roche (2 mentions) Anti rage, by Cell Signaling Technology (1 mentions) Synergytm h4 hybrid multi mode microplate reader, by Agilent Technologies (1 mentions) Luminometer, by Thermo Fisher Scientific (1 mentions)

4 protocols using realtime glo mt luminescent kit

Adenoviral Transduction and Cytotoxicity Assays

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Adenovirus expressing β-galactosidase, APE1/Ref-1, or PPTLS-APE1/Ref-1 was prepared as previously described [24 (link)]. The effect of each adenovirus-mediated protein expression on cell viability and DNA fragmentation of MDA-MB-231, MDA-MB-468, and BT-549 cells was determined using a RealTime-Glo MT luminescent kit (Promega, Madison, WI, USA) and Cell Death Detection ELISA kit (Roche Applied Science, Indianapolis, IN, USA), respectively.
APE1/Ref-1 derived from cells expressing an adenoviral-mediated protein in the culture supernatant or whole-cell lysates was immunoprecipitated using anti-RAGE (Cell Signaling Technology, Beverly, MA, USA), followed by immunoblotting using anti-APE1/Ref-1 or anti-RAGE antibodies as described previously [19 (link)].

Choi S., Lee Y.R., Kim K.M., Choi E, & Jeon B.H. (2022). Dual Function of Secreted APE1/Ref-1 in TNBC Tumorigenesis: An Apoptotic Initiator and a Regulator of Chronic Inflammatory Signaling. International Journal of Molecular Sciences, 23(16), 9021.

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Evaluating FeHAs Effects on HL-1 Cell Viability

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The effect of FeHAs on HL-1 cell viability was analysed using a RealTime-GloMT luminescent kit (Promega) according to the manufacturer's instructions. Briefly, 5 × 10⁴ cells well⁻¹ were seeded in precoated white-walled 96-well plates (1 h, 37°C). Twenty-four hours after plating, HL-1 cells were treated with various concentrations of FeHAs. To continuously monitor the viability of treated HL-1 cells in real time, RealTime-Glo Reagents were added at the same time as FeHAs. Luminescence intensity at the desired time points, indicated in the text, was measured up to 48 h using a microplate reader SynergyTM H4 Hybrid Multi-Mode Microplate Reader (BioTek). Three biological replicates were analysed in triplicate.

Marrella A., Iafisco M., Adamiano A., Rossi S., Aiello M., Barandalla-Sobrados M., Carullo P., Miragoli M., Tampieri A., Scaglione S, & Catalucci D. (2018). A combined low-frequency electromagnetic and fluidic stimulation for a controlled drug release from superparamagnetic calcium phosphate nanoparticles: potential application for cardiovascular diseases. Journal of the Royal Society Interface, 15(144), 20180236.

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Impact of Ac-APE1/Ref-1 on Cell Viability

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The effect of recombinant human Ac-APE1/Ref-1 on the cell viability and DNA fragmentation of MDA-MB-231, MDA-MB-468, BT-549, MCF-7 cells, HMECs, and HUVECs was determined using the RealTime-Glo MT luminescent kit (Promega, Madison, WI, USA) and Cell Death Detection ELISA kit (Roche Applied Science, IN, USA), respectively. Acetylated recombinant human Ac-APE1/Ref-1 was prepared as previously described^{12 (link)}.

Lee Y.R., Park M.S., Joo H.K., Kim K.M., Kim J., Jeon B.H, & Choi S. (2018). Therapeutic positioning of secretory acetylated APE1/Ref-1 requirement for suppression of tumor growth in triple-negative breast cancer in vivo. Scientific Reports, 8, 8701.

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Evaluating UDE Cytotoxicity on HUVECs

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The effect of UDE on the viability of HUVECs was analyzed using a RealTime-Glo MT luminescent kit (Promega, Madison, WI, USA) in an opaque-walled assay plate, according to the manufacturer's instructions. The permeable viability prosubstrate in live cells is reduced to a luciferase substrate by metabolically active cells, while dead cells neither reduce the substrate nor produce a signal. The reduced substrate diffuses from cells into the surrounding culture medium and is then rapidly used by luciferase to produce a luminescent signal, which shows a correlation with viability. HUVECs in the assay-plate were treated with various concentrations of UDE (1–30 μg/mL) for 24 hours. To continuously monitor the viability of the treated HUVECs in real time, the prosubstrate and luciferase were added at the same time as the UDE. Luminescence intensity at the desired time points was measured using a luminometer (Thermo Scientific, Rockford, IL, USA). Each sample was analyzed at least in triplicate.

Lee K.M., Joo H.K., Lee Y.R., Park M.S., Kang G., Choi S., Lee K.H, & Jeon B.H. (2016). Ulmus davidiana ethanol extract inhibits monocyte adhesion to tumor necrosis factor-alpha-stimulated endothelial cells. Integrative Medicine Research, 5(2), 131-139.

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