Human breast tumor tissue microarrays containing 139 analyzable cases of breast carcinoma were purchased from US Biolab (#HBreD139Su01). Tissue specimens were processed as described in our previous publication5 (link). The primary antibodies used for immunohistochemical analysis were antibodies against DGCR8 (1:250, Abcam, #ab90579) and USP51 (1:250, Abcam, #ab121147). Whole-slide images were captured using an Aperio CS2 slide scanner system (Leica Biosystems, Wetzlar, Germany) at a 40× magnification. Immunohistochemical staining was analyzed by using the immunoreactive score (IRS) system. The percentage of positive cells was scored on a scale of 0–4: 0 if 0% of tumor cells were positive, 1 if 1–10% of cells were positive, 2 if 11–50% were positive, 3 if 51–80% were positive, and 4 if 81–100% were positive. Staining intensity was scored on a scale of 0–3 (3 is the strongest). Final IRS score = (score of the staining intensity) × (score of the percentage of positive cells). Score = 0–6 was considered moderate or weak expression and score = 8–12 was considered strong expression.
Ab121147
Manufactured by Abcam
Sourced in Germany
Ab121147 is a lab equipment product. It is a device used for scientific research and analysis purposes. The core function of this product is to perform specific tasks related to laboratory experimentation or data collection.
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2 protocols using ab121147
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Breast Tumor Tissue Microarray Analysis
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Breast Carcinoma Tissue Microarray Analysis
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Human breast tumor tissue microarrays containing 139 analyzable cases of breast carcinoma were purchased from US Biolab (#HBreD139Su01). Tissue specimens were processed as described in our previous publication 5 . The primary antibodies used for immunohistochemical analysis were antibodies against DGCR8 (1:250, Abcam, #ab90579) and USP51 (1:250, Abcam, #ab121147).
Whole-slide images were captured using an Aperio CS2 slide scanner system (Leica Biosystems, Wetzlar, Germany) at a 40 magnification. Immunohistochemical staining was analyzed by using the immunoreactive score (IRS) system. The percentage of positive cells was scored on a scale of 0 to 4: 0 if 0% of tumor cells were positive, 1 if 1%-10% of cells were positive, 2 if 11%-50%
were positive, 3 if 51%-80% were positive, and 4 if 81%-100% were positive. Staining intensity was scored on a scale of 0 to 3 (3 is the strongest). Final IRS score = (score of the staining intensity) × (score of the percentage of positive cells). Score = 0-6 was considered moderate or weak expression and score = 8-12 was considered strong expression.
Whole-slide images were captured using an Aperio CS2 slide scanner system (Leica Biosystems, Wetzlar, Germany) at a 40 magnification. Immunohistochemical staining was analyzed by using the immunoreactive score (IRS) system. The percentage of positive cells was scored on a scale of 0 to 4: 0 if 0% of tumor cells were positive, 1 if 1%-10% of cells were positive, 2 if 11%-50%
were positive, 3 if 51%-80% were positive, and 4 if 81%-100% were positive. Staining intensity was scored on a scale of 0 to 3 (3 is the strongest). Final IRS score = (score of the staining intensity) × (score of the percentage of positive cells). Score = 0-6 was considered moderate or weak expression and score = 8-12 was considered strong expression.
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