Cyclohexane

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom, France, Germany

Cyclohexane is a colorless, flammable cycloalkane with the chemical formula C6H12. It is a common organic solvent used in various laboratory applications.

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Lab products found in correlation

Close spelling variants of this product

Sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (Sulfo-SMCC) (18 citations) Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (4 citations) Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC), (3 citations) Cyclohexane C6H12 (2 citations) Cyclohexane (HPLC grade, (2 citations)

55 protocols using cyclohexane

Ceria Gel Synthesis via Reverse Micelles

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The ceria gels have been prepared by mixing the Ce(OⁱPr)₄ concentrate with the three different reversed micelle mixtures of Triton X-100 (extra pure, Fisher Chemical), Triton X-114 (extra pure, Sigma-Aldrich) and Triton X-45 (extra pure, Sigma-Aldrich) at a Ce/H₂O-molar ratio 1 : 1.^{10 (link)} The different micelle mixtures were prepared by mixing the corresponding Triton-X surfactant and de-ionized water (molar ratio (Triton-X/H₂O) = 0.8) in cyclohexane (99.98%, Fisher Chemical) at a concentration of 1.2 mol kg⁻¹. Then, the concentrated precursor (Ce(OⁱPr)₄) solution was added to the reverse micelles mixtures. After two days and under ambient conditions the sols were transformed to clear gels and aliquots of these gels have been calcined at different temperatures (300 °C, 400 °C, 500 °C and 600 °C) for two hours at a temperature rate of 6 °C min⁻¹. For comparison, aliquots of the 300 °C samples were also calcined at 400 °C, 500 °C and 600 °C at a temperature rate of 6 °C min⁻¹.

Paschalidou P, & Theocharis C.R. (2019). Surface properties of ceria synthesised using Triton-X based reverse microemulsions. RSC Advances, 9(12), 7025-7031.

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Extraction of β-Carotene from Pumpkin Samples

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Dichloromethane (ACS ISO-Reagent, European Pharmacopoeia, 99.9%), acetone (ACS ISO-Reagent, European Pharmacopoeia, 99.8%), methanol (Jeulin, France, 99.5%), acetonitrile (Scharlau Chemie SA, Spain, 99.7%), and cyclohexane (Fisher Chemical, UK, 99%) were used for extraction of β-carotene. Pumpkin samples were collected from Dukem and Debre Berhan (Ethiopia). β-Carotene standard (Sigma-Aldrich, 99%) were obtained from Ethiopian Public Health Institute.

Hagos M., Redi-Abshiro M., Chandravanshi B.S, & Yaya E.E. (2022). Development of Analytical Methods for Determination of β-Carotene in Pumpkin (Cucurbita maxima) Flesh, Peel, and Seed Powder Samples. International Journal of Analytical Chemistry, 2022, 9363692.

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Synthesis and Characterization of Biomimetic Bone Cements

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Triton X-100, tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTS), sodium alginate, gentamicin sulphate, sodium acetate trihydrate, phosphate buffer solution (PBS) tablets, o-phthaldialdehyde reagent solution (OPA), piperazine, and 1,6 hexanediol diacrylate were purchased from Sigma-Aldrich, UK. Cyclohexane, n-hexanol, ammonium hydroxide (35%), diethyl-ether, di-chloro methane (DCM), ethanol, methanol, glacial acetic acid, and iso-propanol were purchased from Fisher Scientific, UK. All reagents were stored according to manufacturer’s guidelines and used as received. The bone cements brands used were Cemex (Tecres S.p.A., Italy) and Palacos R (Heraeus Medical GmbH, Germany).
Acetic acid-sodium acetate buffer (0.1 M, pH 5) was prepared mixing sodium acetate trihydrate (CH₃COONa·3H₂O) (0.1 M) and acetic acid (CH₃COOH) (0.1 M) solutions 3:7 and stirred, with pH checked and adjusted in the range 5.0 ± 0.1. PBS solution (pH 7.4) was prepared according to manufacturer’s guidelines.

Al Thaher Y., Yang L., Jones S.A., Perni S, & Prokopovich P. (2018). LbL-assembled gentamicin delivery system for PMMA bone cements to prolong antimicrobial activity. PLoS ONE, 13(12), e0207753.

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Synthesis and Characterization of cRGDyK Peptide Conjugates

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Cyclo-(Arg-Gly-Asp-D-Tyr-Lys) peptide (cRGDyK) was procured from Peptide International (Louisville, KY). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, cyclohexane and sodium chloride (NaCl) were purchased from Fisher Scientific (Pittsburg, PA). Sunitinib was purchased from LC Laboratories (Woburn, MA). SCM-PEG_5k-Mal was obtained from Creative PEG works (Winston Salem, NC). p-SCN-Bn-NOTA was acquired from Macrocyclics, Inc.(Dallas, TX). NHS-Fluorescein, Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate(TEOS), ammonia (NH₃.H₂O), Igepal CO-520 (NP-5), triethylamine (TEA), (3-Aminopropyl)triethoxysilane (APS), dimethyl sulfoxide (DMSO), cetyltrimethylammonium chloride (CTAC, 25 wt%) and Kaiser test kit were purchased from Sigma-Aldrich (St. Louis, MO). Traut’s Reagent (2-Iminothiolane. HCl) and PBS (1X) were purchased from Thermo Scientific (Rockford, IL). Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.

Chakravarty R., Goel S., Hong H., Chen F., Valdovinos H.F., Hernandez R., Barnhart T.E, & Cai W. (2015). Functionalized Hollow Mesoporous Silica Nanoparticles for Tumor Vasculature Targeting and PET Image-Guided Drug Delivery. Nanomedicine (London, England), 10(8), 1233-1246.

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Synthesis and Formulation of Posaconazole

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Posaconazole was synthesized by Merck & Co. Inc., Kenilworth, NJ, USA; Posaconazole is a BCS Class II triazole medication to treat fungal infections [50 (link),51 (link)]. The polymer HPMCAS-M grade was a kind gift from ShinEtsu (Tokyo, Japan). The solvents methyl ethyl ketone (MEK), tetrahydrofuran (THF), acetone, methyl tert-butyl ether (MTBE), reagent alcohol, cyclohexane, and n-heptane were purchased from Fisher Scientific (Waltham, MA, USA).

Frank D., Schenck L., Koynov A., Su Y., Li Y, & Variankaval N. (2021). Optimizing Solvent Selection and Processing Conditions to Generate High Bulk-Density, Co-Precipitated Amorphous Dispersions of Posaconazole. Pharmaceutics, 13(12), 2017.

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Synthesis of Poly(1-trimethylsilyl-1-propyne)

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Monomer 1-trimethylsilyl-1-propyne was obtained by reacting methylacetylene from the methylacetylene-allene fraction with an alkyl magnesium halide, which is followed by treating the reaction mixture with trimethylchlorosilane [24 ]. Monomer (99.8%) and the solvent cyclohexane (99.8%, Fisher Scientific, Waltham, MA, USA) were distilled three times over calcium hydride in a high-purity argon atmosphere before polymerization. Catalyst NbBr₅ (99.9%, ABCR, Karlsruhe, Germany), cocatalyst Ph₄Sn (>98.0%, TCI Ltd., Tokyo, Japan), and antioxidant Irganox 1076 (Octadecyl β—(3,5-di- tert -butyl-4-hydroxy phenyl)-propionate) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) were used without further purification.
O₂, N₂ with purity at least 99.999%, Ar with purity at least 99.9999%, and CO₂ with purity at least 99.99% were purchased from Linde (Linde GmbH, Pullach, Germany; Linde Gas, Balashiha, Russia).

Shishatskiy S., Makrushin V., Levin I., Merten P., Matson S, & Khotimskiy V. (2022). Effect of Immobilization of Phenolic Antioxidant on Thermo-Oxidative Stability and Aging of Poly(1-trimethylsilyl-1-propyne) in View of Membrane Application. Polymers, 14(3), 462.

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Preparation and Characterization of Graphene-Carbon Black Nanocomposites

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For this study, GNP and CB were received from XG Sciences (type: Grade M; Lansing, MI, USA) and Cabot Corporation (type: Vulcan XC72; Boston, MA, USA), respectively. The nominal particle dimensions and volumetric mass density are 6–8 nm, 25 µm (thickness, diameter) and 2.2 g/cm³ for the graphene nanoplatelets and 50 nm and 1.8 g/cm³ for the carbon black. Poly-dimethyl siloxane was purchased from Dow Corning (type: Sylgard 184; Auburn, MI, USA). The solvents cyclohexane, toluene, chloroform, acetone and isopropanol with 99.9% purity were purchased from Fisher Scientific (Hampton, NH, USA). Using these materials, different bulk nanocomposites were prepared and experiments were conducted as per the test matrix shown in Table 1.

Yang B., Nagarajan B, & Mertiny P. (2021). Characterization of swelling behavior of carbon nano-filler modified polydimethylsiloxane composites. Journal of Elastomers and Plastics, 53(8), 955-974.

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Synthesis and Characterization of Conjugated Polymers

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Methanol (MeOH), hexane, anisole, 1,2-dichlorobenzene, 2,5-dibromo-3-hexylthiophene, chloroform, lithium chloride (LiCl), [1,3-bis(diphenylphosphino)propane]dichloronickel(II) (Ni(dppp)Cl₂) and tert-butylmagnesium chloride (1.0 M in tetrahydrofuran (THF)) were purchased from Sigma-Aldrich and used as received. 5,5′-Dibromo-3,3′-dihexyl-2,2′-bithiophene was purchased from Tokyo Chemical Industry and used as received. Cyclohexane and THF were purchased from Fisher Scientific. THF for polymerizations was distilled over sodium and benzophenone before use. Hexamethyldisilazane (electronic grade) was purchased from Alfa Aesar and was used as received. Octadecyltrichlorosilane was purchased from Acros Organics.

Li X., Wolanin P.J., MacFarlane L.R., Harniman R.L., Qian J., Gould O.E., Dane T.G., Rudin J., Cryan M.J., Schmaltz T., Frauenrath H., Winnik M.A., Faul C.F, & Manners I. (2017). Uniform electroactive fibre-like micelle nanowires for organic electronics. Nature Communications, 8, 15909.

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Synthesis and Characterization of 2,5-Furandicarboxylic Acid Derivatives

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2,5-Furandicarboxylic acid (>98%) was purchased from TCI Europe NV (Zwijndrecht, Belgium). 2-Ethyl-1-hexanol (≥99.6%), stearic acid (95%), anhydrous sodium sulfate (≥99.0%), zinc stearate purum, and Dulbecco’s modified Eagle’s medium—high glucose (DMEM-HG) were supplied by Sigma Aldrich-chemicals Corp. (Sintra, Portugal). Sulfuric acid (96%), sodium chloride (≥99.0%), and deuterated Chloroform (99.8% D) were acquired from Acros Organics (Geel, Belgium). Chloroform and cyclohexane (HPLC grade) were purchased from Fisher Scientific (Porto Salvo, Portugal and Panreac Applichem (Barcelona, Spain), respectively. Activated carbon (granules with density of 2 g cm⁻³) was obtained from VWR Chemicals. Di(2-ethyl-1-hexyl) 1,4-terephthalate (DEHT) (DOTP-168 Eastman) was supplied by Eastman Chemical Company (Madrid, Spain) and PVC resin (VICIR S1200; The K-Fikentscher value of 70, corresponding to number-average molecular weight equal to 59000 and a polydispersity of 1.97) [22 (link)], was provided from CIRES, Lda. (Portugal). All chemicals were used as received, without further purification.

Matos M., Cordeiro R.A., Faneca H., Coelho J.F., Silvestre A.J, & Sousa A.F. (2019). Replacing Di(2-ethylhexyl) Terephthalate by Di(2-ethylhexyl) 2,5-Furandicarboxylate for PVC Plasticization: Synthesis, Materials Preparation and Characterization. Materials, 12(14), 2336.

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Sterol Extraction and Characterization

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All reagents were analytical grade. Cyclohexane and potassium hydroxide were obtained from Fisher Scientific, Co. (Fair Lawn, NJ, USA). Absolute ethanol was obtained from Merck (Merck KGaA, Darmstadt, Germany). Campesterol, cholestane, cholesterol, stigmasterol, and sitosterol standards were obtained from Sigma-Aldrich, Inc. (Sigma Chemical, St. Louis, MO, USA). The trimethylsilyl (TMS) ether derivatives of all sterols were prepared using N,O-bis-[trimethylsilyl]trifluroacetamide reagent (Pierce Chemical Co., Rockford, IL, USA).

Hsu C.C., Kuo H.C, & Huang K.E. (2017). The Effects of Phytosterols Extracted from Diascorea alata on the Antioxidant Activity, Plasma Lipids, and Hematological Profiles in Taiwanese Menopausal Women. Nutrients, 9(12), 1320.

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