Thioacetamide reagent, ≥99.0 % was bought from Sigma-Aldrich, USA. Silymarin was procured from MEPACO- Egypt; high analytical grade chemicals were used during the experimental procedures. Kits used for measurement of serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin levels, albumin, reduced glutathione (GSH) and malondialdehyde (MDA), were procuredfrom Biodiagnostic, Egypt. Alpha-smooth muscle actin (α -SMA) and collagen I were procured from SinoGeneClone Biotech Co., Ltd.
Aspartate transaminase
Manufactured by Biodiagnostic
Aspartate transaminase (AST) is a laboratory test that measures the level of the enzyme aspartate transaminase in the blood. Aspartate transaminase is an enzyme involved in the breakdown of amino acids and is found in various tissues, particularly the liver and heart.
Automatically generated - may contain errors
Lab products found in correlation
Alanine transaminase, by Biodiagnostic (2 mentions)
Albumin, by Biodiagnostic (1 mentions)
Ab155458, by Abcam (1 mentions)
Reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Ab197742, by Abcam (1 mentions)
Oligo dt primer, by Thermo Fisher Scientific (1 mentions)
Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Urea, by Biodiagnostic (1 mentions)
3 protocols using aspartate transaminase
1
Thioacetamide-induced Liver Injury Model
2
Oxidative Stress Biomarkers Protocol
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Aspartate transaminase (AST), alanine transaminase (ALT), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), and total proteins were sourced from Biodiagnostic Company, Giza, Egypt. Reverse transcriptase, DNA ladder (100 bp), Oligo dT primers, SYBR Green PCR Master Mix, and TRIzol were from Invitrogen. The kits to determine total antioxidant activity (cat # TA 2513) were from Biodiagnostic Co. ELISA kits for mouse IL‐1β (ab197742) and myeloperoxidase (ab155458) were from Abcam Co.
3
Biochemical Assays for Liver and Kidney Functions
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Kits for the enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), creatinine, and urea were purchased from Biodiagnostic Co., Egypt. The transaminase activities were determined calorimetrically as a change in absorbance/minute at the wavelength 505 nm, as described by Reitman and Frankel (1957 (link)). Serum ALP activity was detected colorimetrically at the wavelength 510 nm according to Belfield and Goldberg (1971 (link)). Creatinine was detected according to Bartels et al. (1972 ) and urea levels were measured using the method of Fawcett and Scott (1960 (link)). Serum total protein kits were obtained from Spectrum Diagnostics., Al-Obour city, Cairo, Egypt, and determined by the colorimetric method at 546 nm wavelength according to Sapan et al. (1999 (link)).
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