Sgc 7901

Manufactured by BNCC

Sourced in China

The SGC-7901 is a laboratory instrument designed for scientific research purposes. It is a cell culture incubator that maintains optimal temperature, humidity, and atmospheric conditions for cell growth and experimentation. The SGC-7901 provides a controlled environment to support the in vitro cultivation of various cell lines.

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Lab products found in correlation

17 protocols using sgc 7901

Establishing Cisplatin-Resistant Gastric Cancer Cell Line

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The human normal gastric epithelial cell line (GES-1) and GC cell line (SGC7901) were purchased from the BeNa Culture Collection (Shanghai, China). Cells were cultured in RPMI-1640 medium (Hyclone, Logan, UT, United States) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, United States) and 1% penicillin-streptomycin (Solarbio, Beijing, China) at 37°C in a humidified atmosphere containing 5% CO₂.
The cisplatin-resistant adenocarcinoma cell line from human stomach, SGC7901/DDP, was established by exposing parental SGC7901 cells to cisplatin in RMPI-1640 plus 10% FBS by gradually increasing the cisplatin concentration until the cells acquired resistance to 50 μmol/L.

Zhao R., Zhang X., Zhang Y., Zhang Y., Yang Y., Sun Y., Zheng X., Qu A., Umwali Y, & Zhang Y. (2020). HOTTIP Predicts Poor Survival in Gastric Cancer Patients and Contributes to Cisplatin Resistance by Sponging miR-216a-5p. Frontiers in Cell and Developmental Biology, 8, 348.

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Modulating miR-1-3p and CENPF in Gastric Cells

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Normal human gastric epithelial cell line GES1 (BNCC337969), GC cell lines AGS (BNCC338141), SGC-7901 (BNCC100114) and MGC803 (BNCC100665) were procured from BeNa Culture Collection (BNCC). GES1 and MGC803 were grown in Dulbecco’s Modified Eagle Medium (DMEM) + 10% fetal bovine serum (FBS). AGS cell line was cultivated in F-12 complete medium + 10% FBS. SGC-7901 cell line was grown in Roswell Park Memorial Institute (RPMI) complete medium. They were all preserved in standard cultural condition. RiboBio provided miR-1-3p inhibitor, miR-1-3p mimic (miR-mimic), pcDNA3.1-CENPF plasmids (oe-CENPF) encoding CENPF and corresponding negative controls (NCs). These plasmids were transfected to designated cell lines with lipofectamine RNAiMAX (Life Technologies).

Zhou S., Han H., Yang L, & Lin H. (2022). MiR-1-3p targets CENPF to repress tumor-relevant functions of gastric cancer cells. BMC Gastroenterology, 22, 145.

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Authentication and Culture of Human Cancer Cell Lines

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The human cancer cell lines BGC-823 and SGC-7901 were obtained from the BeNa Culture Collection. Furthermore, all cancer cells were authenticated by short tandem repeat (STR profiles are attached in supplemental materials) and tested for mycoplasma contamination. The human cell culture had gained the ethical approval in Zhuhai Hospital Affiliated with Jinan University. Both cell lines were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (FBS, HyClone, Logan, UT, USA), 100 U/mL penicillin, and 100 U/mL streptomycin (Gibco, Grand Island, NY, USA). The cells were grown at 37°C in a humidified incubator (BB15, Thermo Fisher Scientific Inc., Langenselbold, Germany) with 5% CO₂.

He X., Li H., Zhan M., Li H., Jia A., Lin S., Sun L., Du H., Yuan S, & Li Y. (2019). Camellia nitidissima Chi Extract Potentiates the Sensitivity of Gastric Cancer Cells to Paclitaxel via the Induction of Autophagy and Apoptosis. OncoTargets and therapy, 12, 10811-10825.

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Culturing and Treating Human GC Cells

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The human GC cell line SGC7901 was obtained from the Be Na Culture Collection (BNCC, Shanghai, China). Cells were cultured in Dulbecco's modified Eagleʼs medium (DMEM; HyClone, Logan, USA) containing 10% fetal bovine serum (FBS; Biological Industries, Beit HaEmek, Israel) and 1% penicillin-streptomycin (HyClone) and were maintained in a 5% CO₂ incubator at 37°C. Cell passage was performed when the cells reached 80% of confluence.
SGC7901 cells (5 × 105–1 × 106 cells/well) were seeded in 6-well plates and treated with SJZ (DMEM containing 10% SJZ group serum) or NS (DMEM containing 10% control group serum). Cells were harvested at 48 h after treatment for use in the subsequent experiments.

Li X., Chen W., Miao L., Sun H., Gao X., Guo S., Zhang Y., Yang Y, & Guo J. (2022). Antitumor Effect of Si-Jun-Zi Decoction on SGC7901 Gastric Cancer Cells by CMTM2 Activation. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 4675815.

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Gastric Cancer Cell Line Transfection

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The human gastric cancer cell lines (MGC-803, AGS, HGC-27 and SGC-7901) and normal human gastric mucosal epithelial cell GES-1 were purchased from Bena Culture Collection (Beijing, China). DMEM medium (Sigma, St. Louis, MO, USA) plus 10% fetal bovine serum and antibiotics was applied to the culture of gastric cancer cells and normal cells at 37°C with 5% CO₂.
The overexpression vector of circNRIP1 (has_circ_0002711) was generated by inserting into pcDNA3.1 empty vector (pcDNA) (Thermo Fisher Scientific, Wilmington, DE, USA). Small interfering RNA (siRNA) against circNRIP1 (si-circNRIP1) (5ʹ-ACGCACAAAGAAAGAAGTGTT-3ʹ), siRNA negative control (si-NC) (5ʹ-UCUCCGAACGUGUCACGUTT-3ʹ), miR-182 mimic (miR-182) (5ʹ-UUUGGCAAUGGUAGAACUCACACU-3ʹ), miRNA negative control (miR-NC) (5ʹ-CCUGGUAAUGGUAGAAUCUACACU-3ʹ), miR-182 inhibitor (anti-miR-182) (5ʹ-AGUGUGAGUUCUACCAUUGCCAAA-3ʹ) and inhibitor negative control (anti-miR-NC) (5ʹ-UAAUUCAAAAGACUAAAGGAAUCA-3ʹ) were generated by GenePharm (Shanghai, China). These constructed oligonucleotides (20 nM) or vectors (1 μg) were transfected into MGC-803 and AGS cells using Lipofectamine 3000 (Thermo Fisher Scientific). The non-transfected cells were regarded as the mock group. After the transfection for 24 h, cells were collected and used for further experiments.

Liang L, & Li L. (2020). Down-Regulation of circNRIP1 Promotes the Apoptosis and Inhibits the Migration and Invasion of Gastric Cancer Cells by miR-182/ROCK1 Axis. OncoTargets and therapy, 13, 6279-6288.

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Culturing Gastric Cell Lines

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Human GC cell lines (MKN45, AGS and SGC7901) and a normal gastric epithelial cell line (GES1) were purchased from the BeNa Culture Collection (Beijing, China). All the cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, NY, USA) supplemented with 10% foetal bovine serum (FBS) (Sciencell, CA, USA) and 1% penicillin/streptomycin (HyClone, UT, USA) at 5% CO₂ and 37 °C. The IFN-γ used for the experimental treatment of the cells was purchased from PeproTech (NJ, USA).

Yuan J., Tan L., Yin Z., Zhu W., Tao K., Wang G., Shi W, & Gao J. (2019). MIR17HG-miR-18a/19a axis, regulated by interferon regulatory factor-1, promotes gastric cancer metastasis via Wnt/β-catenin signalling. Cell Death & Disease, 10(6), 454.

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Cell Lines for Gastric Cancer Research

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Human normal gastric epithelial cell line GES 1 and gastric cancer cell lines MKN28, SGC-7901 and NCI-N87 were purchased from BeNa Culture Collection (Beijing, China). For cell culture, GES 1 and MKN28 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) with high glucose (Thermo Fisher Scientific, MA, USA), supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific). NCI-N87 and SGC-7901 cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific) with 10% FBS supplement.

Chen W., Wu G., Zhu Y., Zhang W., Zhang H., Zhou Y, & Sun P. (2019). HOXA10 deteriorates gastric cancer through activating JAK1/STAT3 signaling pathway. Cancer Management and Research, 11, 6625-6635.

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Culturing GC and Gastric Cell Lines

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Four GC cell lines (AGS, BGC-823, MKN-45 and SGC-7901) and a gastric epithelial cell line (GES-1) were purchased from BeNa Culture Collection (Beijing China). AGS cells were cultured in Ham's F12 medium (HyClone, GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA), and the remaining cell lines were cultured in Dulbecco's modified Eagle's medium/high glucose medium (HyClone; GE Healthcare Life Sciences) supplemented with 10% FBS at 37°C in 5% CO₂.

Pang K., Ran M.J., Zou F.W., Yang T.W, & He F. (2018). Long non-coding RNA LINC00857 promotes gastric cancer cell proliferation and predicts poor patient survival. Oncology Letters, 16(2), 2119-2124.

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Culturing Human Gastric Cancer Cells

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The human gastric cancer cell line SGC7901 was purchased from the Be Na Culture Collection (BNCC, Shanghai, China) and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone, Logan, UT, USA) containing 10% fetal bovine serum (FBS; Biological Industries, Beit HaEmek, Israel) supplemented with 1% penicillin-streptomycin (HyClone) at 37°C in a humidified incubator containing 5% CO₂.

Guo J., Li X., Miao L., Sun H., Gao X., Guo S., Zhang Y., Cong P, & Chen W. (2021). High-Throughput Sequencing Reveals the Differential MicroRNA Expression Profiles of Human Gastric Cancer SGC7901 Cell Xenograft Nude Mouse Models Treated with Traditional Chinese Medicine Si Jun Zi Tang Decoction. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 6119212.

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Gastric Cancer Cell Line Cultivation

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Human GC cell lines BGC-823 (BNCC100086), MGC-803 (BNCC340396), SGC-7901 (BNCC100114), HGC-27 (BNCC100097), AGS (BNCC102154) and normal gastric cell line GES-1 (BNCC342032) were purchased from BeNa Culture Collection (Beijing, China). All cells were cultured in the RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) as well as 100 U/mL streptomycin (Gibco, Thermo Fisher Scientific, Inc.) and 100 U/mL penicillin (Gibco, Thermo Fisher Scientific, Inc.) under the environment of 5% CO₂ at 37 ℃. The medium was replaced on a regular basis.

Zhou K., Song B., Wei M., Fang J, & Xu Y. (2020). MiR-145-5p suppresses the proliferation, migration and invasion of gastric cancer epithelial cells via the ANGPT2/NOD_LIKE_RECEPTOR axis. Cancer Cell International, 20, 416.

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