Anti nono

Manufactured by Fortis Life Sciences

Anti-NONO is a lab equipment product that targets the NONO protein. It is designed to facilitate the study and analysis of the NONO protein in various research applications.

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Lab products found in correlation

Anti flag, by Merck Group (2 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) Anti vimentin, by Santa Cruz Biotechnology (1 mentions) Anti pabpc1, by Abcam (1 mentions) Anti eif4e, by Fortis Life Sciences (1 mentions) Anti α tubulin, by Santa Cruz Biotechnology (1 mentions) Anti calnexin, by Enzo Life Sciences (1 mentions) Anti pgc 1α, by Novus Biologicals (1 mentions) Anti mhc, by Abcam (1 mentions) Anti myoglobin, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti h3k9me3, by Diagenode (1 mentions) Anti setdb1, by Fortis Life Sciences (1 mentions) Human recombinant vegf a 165, by R&D Systems (1 mentions) Anti dsdna 35i9 dna, by Abcam (1 mentions) Rnase h, by New England Biolabs (1 mentions) Rnase a, by New England Biolabs (1 mentions) Recombinant human tnf α, by Thermo Fisher Scientific (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions)

3 protocols using anti nono

Comprehensive Western Blot Analysis

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Western blotting was performed as described (Elbarbary et al. 2013 (link)) using the following antibodies: anti-STAU1 (Lucas et al. 2018 (link)), anti-FLAG (Sigma), anti-CANX (Enzo Life Sciences), anti-NONO (Bethyl Labs), and anti-STAU2 (Sigma).

Zheng D., Cho H., Wang W., Rambout X., Tian B, & Maquat L.E. (2020). 3′READS + RIP defines differential Staufen1 binding to alternative 3′UTR isoforms and reveals structures and sequence motifs influencing binding and polysome association. RNA, 26(11), 1621-1636.

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Western Blot Analysis of Protein Targets

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Cell lysates were electrophoresed in 8%, 10%, or 12% polyacrylamide gels, and proteins were transferred to a nitrocellulose membrane (Amersham Biosciences) by electroblotting. Proteins were detected using the following antibodies: anti-PGC-1β (Bethyl Laboratories, A302-273A), anti-PGC-1α (Novus, NBP1-04676), anti-Flag (Sigma-Aldrich, A8592), anti-β-actin (Sigma-Aldrich, A2228), anti-CBP80 (Bethyl Laboratories, A301-793A or, alternatively, Choe et al. 2010 (link)), anti-CBP20 (Santa Cruz Biotechnology, sc-48793), anti-PABPC1 (Abcam, ab21060), anti-eIF4E (Bethyl Laboratories, A301-153A), anti-α-Tubulin (Santa Cruz Biotechnology, sc-58666), anti-P62 (BabCO, mAb414), anti-vimentin (Santa Cruz Biotechnology, sc-6260), anti-pSer2-RNAPII (Abcam, ab5095), anti-GAPDH (Cell Signaling Technology, 2118), anti-NONO (Bethyl Laboratories, A300-582A), anti-myoglobin (Santa Cruz Biotechnology, sc25607), anti-MHC (Abcam, ab91506), or anti-calnexin (Enzo, SPA-865F).

Cho H., Rambout X., Gleghorn M.L., Nguyen P.Q., Phipps C.R., Miyoshi K., Myers J.R., Kataoka N., Fasan R, & Maquat L.E. (2018). Transcriptional coactivator PGC-1α contains a novel CBP80-binding motif that orchestrates efficient target gene expression. Genes & Development, 32(7-8), 555-567.

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Analyzing Chromatin Interactions and Modifications

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The following chemicals and concentrations were used: Human recombinant VEGF-A 165 (R&D, 293-VE), Recombinant Human FGF-basic (154 a.a.) (bFGF, Peprotech, 100-18B), RNase A (NEB, EN0531), RNase H (NEB, M0297L), Cycloheximide (Sigma, C1988) and human recombinant TNF-α (Peprotech, 300-01 A). The following antibodies were used: Anti-H3-pan (Diagenode, C15200011), Anti-dsDNA [35I9 DNA] (Abcam, ab27156), Anti-DNA-RNA Hybrid [S9.6] (Kerafast, ENH001), Anti-EPHA2 (Bethyl, A302-025-M), Anti-GAPDH (Sigma, G8795), Anti-HSC70/HSP70 (Enzo Life Sciences, ADI-SPA-820), Anti-NONO (Bethyl, A300-587A), Anti-MPP8 (Bethyl, A303-051A-M), Recombinant Anti-6X His tag® antibody [EPR20547] (Abcam, ab213204, ChIP grade), Anti-H3K9me3 (Diagenode, SN-146-100), Anti-SETDB1 (Bethyl, A300-121A, for chromatin immunoprecipitation; Santa Cruz Biotechnology, ESET (G-4): sc-271488, for Proximity ligation assay) and Anti-ZNF638/NP220 (Bethyl, A301-548A-M).

Leisegang M.S., Bains J.K., Seredinski S., Oo J.A., Krause N.M., Kuo C.C., Günther S., Sentürk Cetin N., Warwick T., Cao C., Boos F., Izquierdo Ponce J., Haydar S., Bednarz R., Valasarajan C., Fuhrmann D.C., Preussner J., Looso M., Pullamsetti S.S., Schulz M.H., Jonker H.R., Richter C., Rezende F., Gilsbach R., Pflüger-Müller B., Wittig I., Grummt I., Ribarska T., Costa I.G., Schwalbe H, & Brandes R.P. (2022). HIF1α-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex. Nature Communications, 13, 6563.

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