CD4+ and CD8+ T cells were individually depleted to investigate the T-cell dependency of the BiCyclA treatment effect. Mice were injected intraperitoneally with 200 µL of sterile saline with 100 µg of antibody every 3 days, beginning 3 days after tumor inoculation. CD4+ T cells were depleted with anti-mouse CD4 (Clone GK1.5, BioXcell, Catalog # BE0003-1). CD8+ T cells were depleted with anti-mouse CD8α (Clone 2.43, BioXcell, Catalog # BE0061). As a control, one group of mice were given rat IgG2b isotype control, anti-keyhole limpet hemocyanin (Clone LTF-2, BioXcell, Catalog # BE0090).
Anti mouse cd8α
Manufactured by BioXCell
Sourced in United States
Anti-mouse CD8α is a laboratory reagent used to detect and quantify CD8α-positive cells in mouse samples. It is a monoclonal antibody that binds specifically to the CD8α subunit, which is expressed on the surface of cytotoxic T cells and a subset of natural killer cells. This product can be used in flow cytometry, immunohistochemistry, and other immunoassays to study the immune system and its response to various stimuli.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cd4, by BioXCell (1 mentions)
Be0061, by BioXCell (1 mentions)
Be0131, by BioXCell (1 mentions)
Be0089, by BioXCell (1 mentions)
Be0087, by BioXCell (1 mentions)
Be0146, by BioXCell (1 mentions)
Anti mouse pd 1, by BioXCell (1 mentions)
Anti mouse ctla 4, by BioXCell (1 mentions)
Smartstrainer, by Miltenyi Biotec (1 mentions)
Tumor dissociation kit, by Miltenyi Biotec (1 mentions)
Hypericin, by Merck Group (1 mentions)
Avaspec 2048x14 spu2 fiberoptic spectrometer, by Avantes (1 mentions)
5 protocols using anti mouse cd8α
1
T-cell Depletion in BiCyclA Treatment
2
Prostate Cancer Immunotherapy Protocol
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At the age of four months, a combination of anti-mouse PD1 (Bio X Cell, BE0146) and anti-mouse CTLA-4 (Bio X Cell, BE0131) was administered at a dose of 5 μg of each antibody per gram of mouse, both antibodies in 100 μL. To eliminate CD8 T cells, anti-mouse CD8α (Bio X Cell, BE0061) was administered at a dose of 100 μg per mouse. As control treatment, a combination of rat IgG2a isotype control (Bio X Cell, BE0089) and polyclonal Syrian hamster IgG (Bio X Cell, BE0087) was used as a control at the same dose. All treatments were administered by intraperitoneal injection every three days for eighteen days. Mice were weighed every three days to discard treatment toxicity. The in vivo response to immune checkpoint blockade or CD8 T cells inhibition was evaluated by measuring each prostate lobe volume one week after the treatment regime was completed.
3
Tumor Immunotherapy Combination Protocol
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All DsiRNAs were synthesized by Integrated DNA Technologies (IDT, Coralville, IA). Primer and probe oligonucleotides used in real-time qPCR detection were synthesized by IDT or Life Technologies (Carlsbad, CA). Monoclonal antibodies (anti-mouse PD-1, anti-mouse CTLA-4, and anti-mouse CD8α) were purchased from Bio X Cell (Lebanon, NH). Tumor dissociation kit and Smart Strainers used to make single-cell suspension were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Monoclonal antibodies used for flow cytometry were purchased from BioLegend (San Diego, CA). DCR-BCAT and Placebo LNPs were prepared as previously described.19 (link) Anti PD-1 antibody, anti-CTLA-4 antibodies, and anti-CD8a antibodies were diluted in PBS and administered intraperitoneally. DCR-BCAT and DCR-Placebo (LNP with chemistry-matched, scrambled CTNNB1 DsiRNA) were given intravenously. LGK-974 was purchased from Selleckchem.com (Houston, TX). LGK-974 was dissolved in DMSO (2%) and Corn Oil (solvents added individually and in order) and was administered orally.
4
CD8+ T Cell Depletion in MC38-luc Tumors
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CD8 + T cells were depleted from MC38-luc orthotopic tumor-bearing mice by injecting 200 µg/100 microliter (µL) PBS anti-mouse CD8α (BioxCell, Lebanon NH, USA) or IgG control s.c. every third day from days 5–19 and monitored by IVIS (Perkin Elmer).
5
Photodynamic Therapy Agents Protocol
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Meso-tetraphenyl chlorin disulphonate (TPCS2a) was obtained from PCI Biotech AS (Oslo Norway) VEGF121/rGel, from now indicated as VEGF121/rGel, was produced as previously described (9 (link)). Hypericin was obtained from Sigma-Aldrich (St Louis, MO, USA) while Anti-mouse CTLA-4 (CD152) and anti-mouse CD8α were obtained from BioxCell (Lebanon, NH, USA). In vitro TPCS2a light exposure was performed with a LumiSource TM lamp (PCI Biotech AS) while TPCS2ain vivo light exposure was done with a 652nm diode laser (CeramOptec GmbH, Bonn, Germany). For Hypericin yellow irradiations were carried out with a custom-made lamp equipped with an array of 1W LEDs (18 (link)). The irradiance was measured to 0.9 mW cm−2 by a photodetector PH100-SiUV coupled to a calibrated optic power meter Gentec-EO SOLO2 (Gentec-EO, Quebec, Canada). The spectrum of the yellow lamp was measured by an irradiance-calibrated AvaSpec-2048x14-SPU2 FiberOptic Spectrometer (Avantes, Apeldoorn, The Netherlands) and peaked at approximately 590 nm. For more information see Supplementary Material
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