The Phospho Explorer Antibody Array (PEX100) was obtained from Full Moon Biosystems (Sunnyvale, CA, USA). Lysates of cPLA2α-knockdown as well as cPLA2α-overexpressing HepG2 cells were used as experimental samples. The detailed procedure was conducted as described previously18 (link). The phosphorylation ratio of each phosphorylation site was calculated based on the following equation: phosphorylation ratio = phosphorylated molecules/unphosphorylated molecules. Phosphorylated proteins were considered as differentially expressed, once an increase (> 1.18) or a decrease (< 0.85) occurred in the expression level ratio in cPLA2α-knockdown HepG2 cells relative to cPLA2α-overexpressing HepG2 cells.
Phospho explorer antibody array pex100
Manufactured by Full Moon BioSystems
Sourced in United States
The Phospho Explorer Antibody Array (PEX100) is a high-throughput tool for the detection and quantification of phosphorylated proteins. It contains 100 highly specific antibodies targeting various phosphorylation sites across different proteins, allowing for the simultaneous analysis of multiple signaling pathways in a single experiment.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using phospho explorer antibody array pex100
1
Phosphorylation Profiling of cPLA2α in HepG2 Cells
2
Profiling Protein Phosphorylation in βCKO Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Phospho Explorer Antibody Array (PEX100) and Signaling Explorer Antibody array (SET100) were manufactured by Full Moon Biosystems. Two groups of two βCKO and two untreated βCKO-ERT2 (WT) control male mice aged 8 to 10 wk were used as experimental replicates. Total protein was extracted from βCKO and control islet lysates, labeled with biotin, conjugated to preblocked microarray slides, and detected with Cy3-streptavidin. Slides were returned to Full Moon BioSystems for scanning. Spot intensities were extracted from the scanned array images using GenePix 5. Background readings from each array were subtracted for each spot, and average median signal was used for analysis. The array was normalized against total average spot intensity on the array.
3
Phosphoproteome Analysis of PANC-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Phospho Explorer Antibody Array (PEX100) was designed and manufactured by Full Moon BioSystems (Sunnyvale, CA, USA). Data were collected and analyzed by Wayen Biotechnologies (Shanghai, China). Cell lysates obtained from R116C and WT PANC-1 cells were biotinylated with an Antibody Array Assay Kit. The phosphorylation ratio was calculated as follows: phosphorylation ratio = phosphorylated value/non-phosphorylated value. Significantly changed phosphoproteins (p < 0.05) over 1.2-fold (up- or downregulation) were included. The related pathways were analyzed in the DAVID online database.
4
Phospho Explorer Antibody Array Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Phospho Explorer Antibody Array PEX100 containing 1,318 well-characterized site-specific antibodies was purchased from Full Moon Biosystems (Sunnyvale, CA, USA). The isolated whole fraction of leukocytes was processed according to the manufacturer’s instructions. Briefly: cells were lysed, labeled by Biotin, placed to glass array, and incubated overnight at 4°C. Cy3-Streptavidin was used for protein visualization, signal to noise ratio was measured on GenePix® Microarray Scanner (4000A; Molecular Devices, USA).
5
Phospho Explorer Antibody Array Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lysates obtained from RAW264.7 cells treated with LPS (200 ng/mL) and with or without coelonin (2.5 μg/mL) were applied to a Phospho Explorer antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA, USA). The microarray contains 1318 antibodies [52 (link)], each of which has two replicates that are printed on a coated glass microscope slide, along with multiple positive and negative controls. The antibody microarray experiment was performed according to the manufacturer’s protocol. The protein phosphorylation level was measured as a ratio of the phospho and unphospho values.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!