Gbx2CreER−IRES−EGFP/+ mice (Chen et al., 2009 (link)) were generously provided by Dr. James Li (University of Connecticut Health Center). Rosa26tdTomato reporter mice (Ai14 allele, Madisen et al., 2010 (link), referred to as Rosa26tdTomato in this manuscript) were purchased from Jackson Laboratories (Stock No. 007908) and TaumGFP(Tau-loxP-STOP-loxP-mGFP-IRES-NLS-LacZ-pA, referred to as mGFP in this manuscript) reporter mice (Hippenmeyer et al., 2005 (link)) were generously provided by Dr. S. Arber (Jax lab, Stock No. 021162, www.informatics.jax.org/accession/MGI:3590682 ). Mice were housed and handled according to Brown University Institutional Animal Care and Use guidelines. Genotyping was done as previously described (Ellisor et al., 2009 (link); Jackson Laboratories).
Taumgfp
Manufactured by Jackson ImmunoResearch
TaumGFP is a fluorescent protein derived from the Taumsia marine organism. It exhibits green fluorescence under appropriate excitation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using taumgfp
1
Transgenic Mouse Models for Neuronal Tracing
2
Transgenic Mouse Experiments with Tamoxifen
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Experiments were performed with 8-to 12-week-old female C57BL/6 (Charles River) mice in accordance with the ethics animal care guidelines of the University of Calgary Animal Care Committee. All transgenic animals used in this study were purchased from Jackson Laboratories (PDGFRa-CreER, 018280; PGC1a-flox, 009666; Tau-mGFP, 021162) and crossed in-house. Genotyping (including primers) was conducted using protocols provided online through Jackson Laboratories. Naive animals were injected intraperitonially with 100 mL of 20 mg/mL tamoxifen (in corn oil) daily for 5 days to induce recombination. Animals were given a minimum 5 day washout period following dosing to ensure that tamoxifen had completely left the system. Animals receiving drug treatment were given double-distilled H 2 O (ddH 2 O) or clemastine (dissolved in ddH 2 O, 10 mg/kg; Tocris Bioscience) daily via gastric gavage.
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