Il 1β elisa kit

Manufactured by BD

Sourced in United States

The IL-1β ELISA kit is a laboratory tool used to measure the concentration of the cytokine interleukin-1 beta (IL-1β) in biological samples. This kit employs the enzyme-linked immunosorbent assay (ELISA) technique to quantify the target analyte.

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Lab products found in correlation

Tnf α elisa kit, by Thermo Fisher Scientific (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Bay11 7082, by Merck Group (1 mentions) Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions) Il 18 elisa kit, by R&D Systems (1 mentions) Pf06650833, by Merck Group (1 mentions) Zyvad ome fmk, by Enzo Life Sciences (1 mentions) Fetal bovine serum (fbs), by MP Biomedicals (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Cytiva (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions) Pro prep solution, by iNtRON Biotechnology (1 mentions)

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IL-1β (109 citations) ELISAs (6 citations)

6 protocols using il 1β elisa kit

Assessing Inflammation and Sympathetic Function After MI

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IL-1β in plasma or myocardial homogenates from the border zone at day 3 after MI was detected with an IL-1β ELISA kit (BD Biosciences, San Diego, CA) according to the manufacturer's instructions.
To assess sympathetic nerve function, norepinephrine levels were measured in the myocardium from the remote zone. The total level of norepinephrine was assessed using a commercial ELISA kit (Noradrenalin ELISA, IBL Immuno-Biological Laboratories Co, Hamburg, Germany).

Lee C.C., Chen W.T., Chen S.Y, & Lee T.M. (2021). Taurine Alleviates Sympathetic Innervation by Inhibiting NLRP3 Inflammasome in Postinfarcted Rats. Journal of Cardiovascular Pharmacology, 77(6), 745-755.

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Cytokine Quantification in LPS-Stimulated N9 Cells

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IL-1β ELISA kit was purchased from B&D systems Inc (cat: # DY401-05). TNF-α ELISA kit was purchased from PEPROTECH Inc (cat: 900-TM54). The N9 cell culture cell were cultured till 80% confluent, and then subjected to 100 ng/ml LPS and treated with 2.8 μM of AMS-17 for 24 h. The supernatant of the N9 cell culture was then harvested immediately after drug treatments and centrifuged at 4 °C to remove cell debris before use. IL-1β and TNF-α ELISA experiments were conducted as manufacture instructed protocol.

Zhang C., Sajith A.M., Xu X., Jiang J., Bowen J.P., Kulkarni A, & Hao J. (2022). Targeting NLRP3 Signaling by a Novel-designed Sulfonylurea Compound for Inhibition of Microglial Inflammation. Bioorganic & medicinal chemistry, 58, 116645.

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Modulation of Inflammatory Cytokine Response

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RAW264.7 cells (5 × 10⁴ cells/mL) were incubated with or without RGSF (2.5 μg/mL, 5 μg/mL, 10 μg/mL, and 20 μg/mL) for 10 min and irradiated (10 Gy) using a blood γ irradiator and incubated at 37 °C for 24 h. Cells were then washed twice with PBS. Cells were incubated with or without RGSF (2.5 μg/mL, 5 μg/mL, 10 μg/mL, and 20 μg/mL) for 10 min and stimulated with LPS (0.1 μg/mL) for 24 h. Cytokine levels in the culture supernatant were evaluated using an IL-1β ELISA kit following the manufacturer's protocol (BD, Franklin Lakes, CA, USA).

Lee Y.J., Han J.Y., Lee C.G., Heo K., Park S.I., Park Y.S., Kim J.S., Yang K.M., Lee K.J., Kim T.H., Rhee M.H, & Kim S.D. (2014). Korean Red Ginseng saponin fraction modulates radiation effects on lipopolysaccharide-stimulated nitric oxide production in RAW264.7 macrophage cells. Journal of Ginseng Research, 38(3), 208-214.

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NLRP3 Inflammasome Activation Pathway Protocol

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Roswell Park Memorial Institute (RPMI) 1640 medium was purchased from Hyclone Laboratories (South Logan, UT, USA). Fetal bovine serum (FBS) was obtained from MP Biomedicals (Santa Ana, CA, USA). Opti-MEM, glutamine and penicillin/streptomycin were the products of Gibco (Grand island, NY, USA). Isoliquiritigenin (1), phorbol 12-myristate 13-acetate (PMA), phenylmethylsulfonyl fluoride (PMSF), polyvinylidene fluoride (PVDF), PF 06650833 (IRAK4 inhibitor), Bay 11-7082 (NF-κB inhibitor) and protease inhibitor cocktail were purchased from Sigma-Aldrich (St. Louis, MO, USA). Suberic acid bis (3-sulfo-N-hydroxysuccinimide ester) sodium salt (BS³) was bought from BioVision (Milpitas, CA, USA). Lactate dehydrogenase (LDH) cytotoxicity WST assay kit and Z-YVAD(Ome)-FMK (caspase-1 inhibitor) were obtained from Enzo Life Sciences (Farmingdale, NY, USA). Pro-prep solution was obtained from iNtRON Biotechnology (Seongnam, Korea). All primary antibodies relating NLRP3 inflammasome and signaling pathway and secondary antibodies were bought form Cell Signaling Technology (Minneapolis, MN, USA). Primary anti-β-actin antibody was obtained from Bethyl Laboratories (Montgomery, TX, USA). IL-1β ELISA kit was purchased from BD Biosciences (San Jose, CA, USA). IL-18 ELISA kit was bought from R&D Systems (Minneapolis, MN, USA).

Choi H.R., Lim H., Lee J.H., Park H, & Kim H.P. (2021). Interruption of Helicobacter pylori-Induced NLRP3 Inflammasome Activation by Chalcone Derivatives. Biomolecules & Therapeutics, 29(4), 410-418.

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Quantification of IL-1β and LPS

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The concentration of culture supernatants and serum of IL-1β were measured by IL-1β ELISA Kit (BD Biosciences-CA, for human and R&D systems, Minneapolis, MN, United States, for mice), and the concentration of culture supernatants of LPS was measured by LPS ELISA Kit (EXPANDBIO) according to manufacturer’s instructions.

Pan P., Zhang Q., Liu W., Wang W., Yu Z., Lao Z., Zhang W., Shen M., Wan P., Xiao F., Shereen M.A., Zhang W., Tan Q., Liu Y., Liu X., Wu K., Liu Y., Li G, & Wu J. (2019). Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage. Frontiers in Microbiology, 10, 2637.

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Measuring IL-1β Secretion in BMDMs

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Supernatants were collected from BMDMs after treatment, and Il-1β concentrations were measured by using Il-1β ELISA kit (BD Biosciences, Cat. #559603) according to manufacturer's protocol.

Dai L., Bhargava P., Stanya K.J., Alexander R.K., Liou Y.H., Jacobi D., Knudsen N.H., Hyde A., Gangl M.R., Liu S, & Lee C.H. (2017). Macrophage alternative activation confers protection against lipotoxicity-induced cell death. Molecular Metabolism, 6(10), 1186-1197.

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