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Sc 1000 orius ccd camera

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The SC 1000 ORIUS CCD camera is a high-performance digital imaging device designed for scientific and industrial applications. It features a large-format CCD sensor that captures high-resolution images with excellent sensitivity and low noise. The camera is capable of capturing detailed images with a wide dynamic range, making it suitable for a variety of imaging tasks.

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Lab products found in correlation

Jem 1400 transmission electron microscope, by JEOL (3 mentions) Tecnai g2 spirit electron microscope, by Thermo Fisher Scientific (2 mentions) Quemesa, by Olympus (2 mentions)

3 protocols using sc 1000 orius ccd camera

1

Transmission Electron Microscopy of Small Extracellular Vesicles

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Samples were mounted on 300 mesh formvar copper grids, stained with uranyl acetate 1%, and examined with a Jeol JEM 1400 transmission electron microscope (Tokyo, Japan). Images were digitally recorded using a Gatan SC 1000 ORIUS CCD camera (Warrendale, Pennsylvania), and photomontages were performed with Adobe Photoshop CS software (Adobe Systems, San Jose, California), at the Institute for Molecular and Cell Biology (IBMC) of the University of Porto, Portugal.
For CD63 immunogold labeling, sEV were prepared according to Thery et al.12 sEV were fixed with 2% PFA and underwent single immunogold labeling with protein A conjugated to gold particles 10 or 15 nm in diameter (Cell Microscopy Center, Department of Cell Biology, Utrecht University, Netherlands). Grids were analyzed on a Tecnai Spirit G2 electron microscope (FEI, Eindhoven, The Netherlands) and digital acquisitions were made with a 4k CCD camera (Quemesa, Olympus, Muenster, Germany).
Cardoso R.M., Rodrigues S.C., Gomes C.F., Duarte F.V., Romao M., Leal E.C., Freire P.C., Neves R, & Simões‐Correia J. (2021). Development of an optimized and scalable method for isolation of umbilical cord blood‐derived small extracellular vesicles for future clinical use. Stem Cells Translational Medicine, 10(6), 910-921.
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2

Transmission Electron Microscopy of SLNs

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In an attempt to determine and evaluate the morphology of the SLNs developed, samples were analyzed by transmission electronic microscopy (TEM). The samples were mounted on 300 mesh form var copper grids, stained with uranyl acetate, and were examined using a Jeol JEM 1400 transmission electron microscope (Tokyo). Images were digitally recorded using a Gatan SC 1000 ORIUS CCD camera (Warrendale, PA, USA), and photomontages were performed using Adobe Photoshop CS software (Adobe Systems, San Jose, CA).
Neves A.R., Queiroz J.F, & Reis S. (2016). Brain-targeted delivery of resveratrol using solid lipid nanoparticles functionalized with apolipoprotein E. Journal of Nanobiotechnology, 14, 27.
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3

Characterization of Extracellular Vesicles by TEM

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Samples were mounted on 300 mesh formvar copper grids, stained with uranyl acetate 1 %, and examined with a Jeol JEM 1400 transmission electron microscope (Tokyo, Japan).
Images were digitally recorded using a Gatan SC 1000 ORIUS CCD camera (Warrendale, PA), and photomontages were performed with Adobe Photoshop CS software (Adobe Systems, San Jose, CA), at the Institute for Molecular and Cell Biology (IBMC) of the University of Porto, Portugal.
For CD63 immunogold labelling, sEV were prepared according to Thery C. et al. (12) . sEV were fixed with 2 % PFA and underwent single immunogold labeling with protein A conjugated to gold particles 10 nm or 15 nm in diameter (Cell Microscopy Center, Department of Cell Biology, Utrecht University, Netherlands). Grids were analyzed on a Tecnai Spirit G2 electron microscope (FEI, Eindhoven, The Netherlands) and digital acquisitions were made with a 4k CCD camera (Quemesa, Olympus, Muenster, Germany).
Cardoso R.M.S., Rodrigues S.C., Gomes C.F., Duarte F.V., Romao M., Leal E.C., Freire P.C., Neves R.P.d., & Simões‐Correia J. (2020). Development of an optimized and scalable method for isolation of umbilical cord blood-derived small extracellular vesicles for future clinical use.
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