Western blots were performed in triplicate as previously described [16 (link)]. The following antibodies were used for Western blot: rabbit anti-Nurr1 polyclonal antibody (1:500, Santa Cruz, CA, USA), rabbit anti-NLK polyclonal antibody (1:250, Abcam, Cambridge, MA, USA), rabbit anti-NF-κB p65 polyclonal antibody (1:200, Santa Cruz, CA, USA), mouse anti-IκBα monoclonal antibody (1:1000, Abcam, Cambridge, MA, USA), mouse anti-CREB monoclonal antibody (1:500, Santa Cruz, CA, USA), rabbit anti-phospho-CREB polyclonal antibody (Ser133; 1:100, Santa Cruz, CA, USA), mouse anti-β-tubulin monoclonal antibody (1:500, Santa Cruz, CA, USA), rabbit anti-Histone H3 polyclonal antibody (1:250, Santa Cruz, CA, USA), mouse anti-β-actin monoclonal antibody (1:1000, Santa Cruz, CA, USA) and mouse anti-CBP monoclonal antibody (1:500, Abcam, Cambridge, MA, USA). Band density was measured with Image J software and normalized against internal control levels.
Mouse anti β tubulin monoclonal antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States, Japan
The Mouse anti-β-tubulin monoclonal antibody is a primary antibody that specifically recognizes the β-tubulin protein in mouse samples. It can be used for various research applications requiring the detection of β-tubulin.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti nlk polyclonal antibody, by Abcam (1 mentions)
Mouse anti cbp monoclonal antibody, by Abcam (1 mentions)
Rabbit anti nurr1 polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti nf κb p65 polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Mouse anti β actin monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Pierce 660 nm protein assay kit, by Thermo Fisher Scientific (1 mentions)
Bio1d software, by Vilber (1 mentions)
Luminata forte hrp substrate, by Merck Group (1 mentions)
Trans blot sd semi dry transfer cell, by Bio-Rad (1 mentions)
Goat anti actin polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti vinculin monoclonal antibody, by Abcam (1 mentions)
Mouse monoclonal anti e cadherin antibody, by BD (1 mentions)
Rabbit anti snail polyclonal antibody, by Abcam (1 mentions)
4 protocols using mouse anti β tubulin monoclonal antibody
1
Western Blot Quantification Protocol
2
Quantitative Western Blot Analysis of hOTC Expression
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hOTC protein expression in Hep3B cell lysates or liver specimens was measured by standard western blotting. Total protein concentrations were determined by Pierce 660 nm Protein Assay Kit (ThermoFisher Scientific). Samples were separated by 10% SDS-PAGE gel and transferred to nitrocellulose membranes by Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated with mouse anti-hOTC monoclonal antibody (Cat# TA802590, OriGene, Rockville, MD) at 1:20,000, mouse anti-β-tubulin monoclonal antibody (Cat# sc-53140, Santa Cruz Biotechnology, Dallas, TX) at 1:5,000, and mouse anti-HSP90AB1 (HSP90) monoclonal antibody (Cat# TA500494, OriGene) at 1:2,000. The signals were visualized by chemiluminescence using LuminataForte HRP substrate (Merck Millipore) with a FUSION (Vilber, Paris, France). The densitometry of each band was quantified by BIO-1D Software (Vilber).
3
Immunostaining for Astrocytes and Tubulin
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Mouse monoclonal anti-GFAP antibody (Cat. No. 36700) was purchased from Cell Signaling. Rabbit polyclonal anti-GS (C20) antibody (Cat. No. sc-6640) was purchased from Santa Cruz Biotechnology, Inc. Mouse anti-β-tubulin monoclonal antibody (Cat. No. T4026) was purchased from Sigma.
4
Immunoblotting of Epithelial-Mesenchymal Transition Markers
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Goat-anti Actin polyclonal antibody (Santa Cruz Biotechnology, Inc., Dallas, TX), rabbit-anti α-Actinin polyclonal antibody (Cell Signaling Technology Japan K.K., Tokyo, Japan), mouse-anti β-tubulin monoclonal antibody (Santa Cruz Biotechnology), mouse-anti E-cadherin monoclonal antibody (BD Biosciences), rabbit-anti N-cadherin polyclonal antibody (Santa Cruz Biotechnology), rabbit-anti S100A4 polyclonal antibody (Merck Millipore, Billerica, MA), rabbit-anti SNAIL polyclonal antibody (Abcam plc, Cambridge, UK), rabbit-anti Talin polyclonal antibody (Santa Cruz Biotechnology), mouse-anti Twist 1 monoclonal antibody (Santa Cruz Biotechnology), rabbit-anti Vinculin monoclonal antibody (Abcam plc), mouse-anti Vimentin monoclonal antibody antibody (Santa Cruz Biotechnology) were used as primary antibodies. Horseradish peroxidase (HRP) linked anti-goat, anti-mouse, and anti-rabbit antibodies (Santa Cruz Biotechnology) were used as secondary antibodies.
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