Total RNA was isolated with RNeasy kit (Qiagen, Carlsbad, CA). Samples were prepared in biological duplicates. Equal amount from each sample was subjected to biotinylation using BioArray High Yield RNA Transcript Labeling System (Enzo Life Sciences, Farmingdale, NY, USA). Equal amount cRNA from each sample was labeled, purified and fragmented by using GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, CA, USA). Following the manufacturer’s protocols, the Human Genome U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA) was applied for gene expression analysis (UCLA Clinical Microarray Core Facility).
Bioarray high yield rna transcript labeling system
Manufactured by Enzo Life Sciences
Sourced in United States
The BioArray High Yield RNA Transcript Labeling System is a laboratory equipment designed to generate labeled RNA transcripts from DNA templates. It offers a streamlined workflow for producing high-yield, labeled RNA samples suitable for various downstream applications.
Automatically generated - may contain errors
2 protocols using bioarray high yield rna transcript labeling system
1
Genome-wide gene expression analysis
2
RNA Extraction and Microarray Analysis
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