Whole blood and intratumoural immune cells were stained with CD56-FITC-Viobright, NKG2A-APC, CCR5-FITC, CCR1-APC, CCR3-PE (Miltenyi Biotec), CD3-APC-Cy7, NKp30-BV421, NKp46-PE-Cy7, NKG2D-PE-Cy5, PD-1-PE-Cy7, TIGIT-PE-Cy5, CD69-BV510 (BioLegend). Red blood cells were lysed using BD Lysing Solution (BD Biosciences) as per manufacturer’s instructions. NK cells were quantified as CD56+CD3− cells within the lymphocyte gate. Cells were acquired using the CANTO II (BD Biosciences) flow cytometer and analysed using FlowJo software (Tree Star).
Tigit pe cy5
Manufactured by BioLegend
TIGIT-PE-Cy5 is a fluorescence-labeled antibody that binds to the TIGIT protein. TIGIT is an inhibitory receptor expressed on various immune cells, including T cells and natural killer cells. The PE-Cy5 fluorescent label allows for the detection and analysis of TIGIT-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Pd1 pe cy7, by BioLegend (2 mentions)
Cd56 fitc viobright, by Miltenyi Biotec (2 mentions)
Nkg2a apc, by Miltenyi Biotec (2 mentions)
Nkp30 bv421, by BioLegend (2 mentions)
Nkp46 pe cy7, by BioLegend (2 mentions)
Nkg2d pe cy5, by BioLegend (2 mentions)
Cd69 bv510, by BioLegend (2 mentions)
Canto 2, by BD (2 mentions)
Cd3 apc cy7, by BioLegend (1 mentions)
Ccr5 fitc, by Miltenyi Biotec (1 mentions)
Ccr1 apc, by Miltenyi Biotec (1 mentions)
Ccr3 pe, by Miltenyi Biotec (1 mentions)
Lysing solution, by BD (1 mentions)
Trail apc, by BioLegend (1 mentions)
Fasl bv421, by BioLegend (1 mentions)
Cd3 apc cy7, by Miltenyi Biotec (1 mentions)
Cd71 pe cy7, by BioLegend (1 mentions)
2 protocols using tigit pe cy5
1
Quantifying NK Cell Markers in Whole Blood and Tumors
2
NK Cell Profiling in Obese OAC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PBMC were isolated from non-cancer controls by density gradient centrifugation and seeded at a density of 1 × 106 cells/ml RPMI supplemented with 10% FBS and 1% pen/strep. Cells were treated with ACM or TCM from non-obese or obese OAC patients for 2 or 24 h. Cells were stained with CD56-FITC-Viobright, NKG2A-APC (Miltenyi Biotec), CD3-APC-Cy7, CD71-PE-Cy7, CD36-PerCP-Cy5.5, NKp30-BV421, NKp46-PE-Cy7, NKG2D-PE-Cy5, PD-1-PE-Cy7, TIGIT-PE-Cy5, CD69-BV510, TRAIL-APC and FasL-BV421 (BioLegend). NK cells were quantified as CD56+CD3− cells within the lymphocyte gate. Cells were acquired using the CANTO II (BD Biosciences) flow cytometer and analysed using FlowJo software (BD Biosciences).
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