Sw1116

Colon cancer cell lines LOVO, HCT116, Caco-2, HT29, SW480, and SW1116 were obtained from American Type Culture Collection (ATCC) and subcultured and preserved by Shanghai Institute of Digestive Surgery; normal human colon mucosal epithelium cell NCM460 was kindly donated by Jingqing Zeng (Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; the donator bought NCM460 cell line from INCELL Corporation, LLC, San Antonio, TX, USA). LOVO was cultured in F-12K Nutrient Mixture Medium (Corning Cellgro®, MA, USA), HCT116 and HT29 in McCoy's 5A (Corning Cellgro®), SW480 and SW1116 in Leibovitz' L-15 (Corning Cellgro®), and NCM460 in DMEM (Corning Cellgro®). All culture medium above was supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA). Caco-2 was cultured in Minimum Essential Medium (Corning Cellgro®) with 20% FBS. Cells were placed in the incubator (Heracelles, Germany) at 37°C, in a humidified atmosphere with 5% CO₂.

Human colorectal cancer cell lines, SW480 and SW1116, were purchased from American Type Culture Collection (ATCC). SW480 and SW1116 were cultured in Leibovitz’ L-15 Medium (Corning Cellgro^®, Manassas, VA, USA) with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 2 mM l-glutamine. Cells were maintained at 37°C/5% CO₂ in a humidified incubator.