D isoleucine

Manufactured by Merck Group

Sourced in United States

D-Isoleucine is a pure, fine crystalline powder that serves as a chemical reagent for laboratory applications. It is a non-proteinogenic amino acid that can be used in various research and analytical procedures.

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Lab products found in correlation

Close spelling variants of this product

Isoleucine

3 protocols using d isoleucine

Amino Acid Synthesis and Characterization

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d-Alanine, d-Arginine, d-Asparticacid, d-Asparagine, d-Glutamicacid, d-Histidine, d-Isoleucine, d-Leucine, d-Lysine, d-Methionine, d-Phenylalanine, d-Proline, d-Serine, d-Threonine, d-Tryptophan, d-Tyrosine, d-Valine, KBr (spectral grade), bovine serum albumin (BSA), glutathione and 3,3′,5,5′-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). d-Cystine and d-Glutarnine were purchased from Aladdin Reagent Co, Ltd. (Shanghai, China). All other chemicals and reagents were of analytical grade. All aqueous solutions were prepared with Milli-Q water.

Jiang N., Zhang C., Li M., Li S., Hao Z., Li Z., Wu Z, & Li C. (2021). The Fabrication of Amino Acid Incorporated Nanoflowers with Intrinsic Peroxidase-like Activity and Its Application for Efficiently Determining Glutathione with TMB Radical Cation as Indicator. Micromachines, 12(9), 1099.

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Preparation of Amino Acid Solutions

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D-methionine, D-tryptophan, D-leucine, D- or L-cysteine, D- or L-aspartic acid, D- or L-glutamic acid, D-alanine, D-threonine, D-valine, D-isoleucine, D-glutamine, D-phenylalanine, D-histidine, D-proline, D-lysine, D-arginine, and glycine (Sigma-Aldrich, St. Louis, MO, USA) were all prepared at a concentration of 40 mM, and D-tyrosine was prepared at a concentration of 0.8 mM due to its low solubility. Nisin powder (2.5% purity, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in a solution at pH 2 to obtain a stock solution with a concentration of 1 g/L.

Tong Z., Zhang L., Ling J., Jian Y., Huang L, & Deng D. (2014). An In Vitro Study on the Effect of Free Amino Acids Alone or in Combination with Nisin on Biofilms as well as on Planktonic Bacteria of Streptococcus mutans. PLoS ONE, 9(6), e99513.

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Peptide-Aldehyde Compound Structural Analysis

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The structure of central part of the peptide-aldehyde compound was still ambiguous, therefore partial amino acid hydrolysis of peptide-aldehyde compounds was performed by dissolving peptide in HCl and hydrolysis at 105 °C (Fig. S1) (26 (link)). The acid was diapered, and the residue solubilized in water. D-Leucine, L-Leucine, D-Isoleucine, Leupeptin, and L-Isoleucine (DAA-20, Sigma Aldrich, Germany) were used as a standard. The amino acid fragmentation products were analyzed with LC-MS software version 2.1.

Nowruzi B., Wahlsten M, & Jokela J. (2019). A Report on Finding a New Peptide Aldehyde from Cyanobacterium Nostoc sp. Bahar M by LC-MS and Marfey’s Analysis. Iranian Journal of Biotechnology, 17(2), e1853.

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