Selection buffer

All ssDNA oligonucleotides were synthesized and purified by high-performance liquid chromatography (HPLC) by Sangon Biotechnology Co., Ltd. (Shanghai, China). Gymnodimine-A (GYM-A), brevetoxin (BTX), spirolide (SPX), and pinnatoxin (PnTX) were purchased from the National Research Council Canada (Halifax, NS, Canada). Okadaic acid (OA), dinophysistoxin (DTX), palytoxin (PLTX), gonyautoxin (GTX), saxitoxin (STX), microcystin-LR (MC-LR), and nodularin-R (NOD-R) were purchased from Taiwan Algal Science Inc. (Taiwan, China). Dynabeads^TM M-270 streptavidin (diameter 2.8 µm) and the Qubit^® ssDNA assay kits were purchased from Thermo Fisher Scientic (Chelmsford, MA, USA). GoTaqHot^® Start Colorless Master Mix was purchased from Promega Corporation (Fitchburg, WI, USA). QIAEX^® II gel extraction kits were purchased from Qiagen (Frankfurt, Germany). Super streptavidin (SSA) sensor biosensors were purchased from ForteBio (Shanghai, China). Selection Buffer (20 mM pH 7.6 Tris-HCl, 100 mm NaCl, 2 mM MgCl₂, 5 mM KCl, and 1 mM CaCl₂) was purchased from Tiandz (Beijing, China). Selection Buffer was also used in biolayer interferometry (BLI) experiments. All reagents were of analytical grade and were used without further purification or treatment unless specified. All solutions were prepared with ultrapure water.

All ssDNA oligonucleotides were synthesized and purified by high-performance liquid chromatography (HPLC) by Sangon Biotechnology Co., Ltd. (Shanghai, China). DA and KA were purchased from the National Research Council Canada (Halifax, NS, Canada). OA, STX, TTX, NOD-R, dinophysistoxin (DTX), and gonyautoxin (GTX) were purchased from Taiwan Algal Science Inc. (Taiwan, China). Dynabeads^TM MyOne^TM Streptavidin C1 (diameter 1.0 μm) and the Qubit^® ssDNA assay kits were purchased from Thermo Fisher Scientific (Chelmsford, MA, USA). GoTaqHot^® Start Colorless Master Mix was purchased from Promega Corporation (Fitchburg, WI, USA). QIAEX^® II gel extraction kits were purchased from Qiagen (Frankfurt, Germany). Super streptavidin (SSA) sensor biosensors were purchased from Sartorius (Shanghai, China). Selection Buffer (pH 7.4, 8 g/L NaCl, 2.8975 g/L Na₂HPO₄, 0.1 g/L MgCl₂·6H₂O, 0.2 g/L KH₂PO₄, 0.2 g/L KCl, and 0.1 g/L CaCl₂) was purchased from Tiandz (Beijing, China). All reagents were of analytical grade and were used without further purification or treatment unless otherwise specified. All the solutions were prepared with ultrapure water.