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Alexa fluor 488 anti igg

Manufactured by Thermo Fisher Scientific
Sourced in Japan

Alexa Fluor 488 anti-IgG is a fluorescently labeled secondary antibody that binds to immunoglobulin G (IgG) antibodies. It is designed for use in various immunoassay and cellular imaging applications.

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2 protocols using alexa fluor 488 anti igg

1

Pluripotency Marker Expression in hiPSCs

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hiPSC colonies were fixed in paraformaldehyde and permeabilized in Triton X-100 prior to Oct4 and Nanog staining. Cells were then stained with secondary antibody Alexa Fluor 488 anti- IgG (Molecular Probes). DAPI staining was performed to visualize the nucleus (Molecular probes). Cells were visualized using the Olympus IX81 fluorescence microscope and images were captured with using the Q Capture software.
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2

Immunofluorescent labeling of α-internexin

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The fixation of culture cells was performed in 0.1% glutaraldehyde containing phosphate saline buffer (pH 7.4). This was followed by blocking with Blocking One (Nacalai Tesque) and an incubation with the anti-α-internexin antibody (1:500, Abcam, Tokyo, Japan, ab10830) and then with Alexa Fluor 488 anti-IgG (Molecular Probes, Eugene, OR, United States).
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