Eds inca 350

The commercial 45S5 BG and Cu-MBGN powders were sputter-coated with 5 nm Au/Pd layer (90% Au/10% Pd), evaporated with argon plasma at 6 kV using the Precision Etching Coating System (Model 682, Gatan Inc., Pleasanton, CA, USA).
FE-SEM images of prepared samples were taken with a scanning electron microscope JSM7000F (JEOL Ltd., Tokyo, Japan) linked to the energy-dispersive X-ray analyzer EDS/INCA 350 (Oxford Instrument, Abingdon, UK).

Morphologies of the samples were investigated by field emission scanning electron microscopy (FE-SEM), model JSM-7000F manufactured by JEOL Ltd., Tokyo. Japan using an accelerating voltage of 10 kV, equipped with an energy dispersive X-ray spectrometer (EDS), EDS/INCA 350 (energy-dispersive X-ray analyser) manufactured by Oxford Instruments Ltd., which was used to prove the presence of barium in the samples.
The presence of Ba-species and anatase TiO₂ was investigated by the means of X-ray diffraction analysis (XRD) using the Siemens D5000 diffractometer equipped with a Cu anode, Goebel mirror and graphite monochromator in front of the point detector. The experiments were performed in grazing incidence geometry with an incidence angle of 1.3° with respect to the film surface plane.

Field emission scanning electron microscope (FE-SEM) JSM-7000F (JEOL) was used for the observation of particle morphology. The FE-SEM was connected to the Oxford Instruments EDS/INCA 350 energy dispersive X-ray analyzer for elemental analysis. Samples dispersed at an appropriate concentration were cast onto a glass sheet at room temperature and imaged. The size distribution was determined using Image-J (Media Cybernetics Inc., Rockville, USA) by measuring diameters of at least 500 MSNs based on FE-SEM images and presented as a histogram of the nanoparticle diameters.

The previously prepared material sample discs were selected for the analysis (n = 5 per material). After the incubation period, the set material discs were coated with carbon under a CC7650 SEM Carbon Coater Unit (Quorum Technologies Ltd.). The superficial element distribution of the coated discs was then individually examined in a scanning electron microscopy (SEM) unit (Jeol 6100 EDAX; Jeol Inc.) attached to an energy dispersive spectroscopy (EDS) system (INCA 350 EDS; Oxford Instruments) for the elemental analysis.

Disc-shaped specimens (10 mm in diameter and 2 mm high) were prepared and immersed in Hank’s Balanced Salt Solution (HBSS) for a total of 28 days. Following immersion, the samples were taken out and placed in acetone for 48 h, and dessicated in a vacuum desiccator for an additional 24 h. Subsequently, the surfaces of the samples were ground with progressively finer diamond discs using an automatic polishing machine. Both control and experimental groups were coated with carbon using a CC7650 SEM Carbon Coater Unit (Quorum Technologies Ltd.). The superficial surface morphology and the element distribution of the coated discs was individually examined at two time intervals of one day and 28 days, using a scanning electron microscopy (SEM) unit (Jeol 6100 EDAX; Jeol Inc.) at 150X magnification, coupled with an energy-dispersive spectroscopy (EDS) system (INCA 350 EDS; Oxford Instruments) for elemental analysis.