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Atr tensor 37 spectrophotometer

Manufactured by Bruker
Sourced in Germany

The ATR-Tensor 37 is a Fourier Transform Infrared (FTIR) spectrophotometer designed for attenuated total reflectance (ATR) measurements. The instrument is capable of acquiring infrared spectra of solid, liquid, and gas samples.

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5 protocols using atr tensor 37 spectrophotometer

1

NMR, IR, and ESI-MS Characterization of Compounds

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NMR spectra were recorded on an NMR spectrometer (BRUKER, Zürich, Switzerland) operating at 600 MHz with cryoprobe prodigy (150 MHz for 13C; chemical shift (δ) = ppm; coupling constants (J) = Hz). Infrared (IR) spectra were recorded on an ATR-Tensor 37 spectrophotometer, Bruker (Ettlingen, Baden-Württemberg, Germany). ESI-MS spectra were recorded on a mass spectrometer (Waters Quattro Premier XE, Waters, Milford, MA, USA). For thin-layer chromatography (TLC, silica gel 60F-254, Merck, Darmstadt, Hesse, Germany), precoated aluminum sheets were used. TLC plates were visualized under UV light at 254 and 366 nm and mostly sprayed with the ceric sulfate (Ce(SO4)2) reagent followed by heating with heating gun.
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2

Spectroscopic Characterization of Organic Compounds

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ESI-HRMS (positive) spectra were documented on Agilent Technologies (6530, Accurate Mass Q-TOF LC/MS). ATR-FTIR (solid) spectra were recorded on a Bruker, ATR-Tensor 37 spectrophotometer with wave numbers (ν) in cm−1. Optical rotations were recorded on a KRUSS P3000 polarimeter purchased from A. Kruss Optronic, Germany. The 1H (600 MHz) and 13C (150 MHz) NMR spectra were measured on BRUKER AVANCE NMR spectrometers (600 MHz) using solvent peaks (CDCl3, δH: 7.26; δC: 77.0), (CD3OD, δH: 4.87; δC: 48.5) as internal reference. Data were reported in the following order: multiplicities are indicated as s = singlet, m = multiplet, t = triplet, dd = doublet of doublet, d = doublet; chemical shift (δ) in ppm; coupling constants (J) are in hertz (Hz). Column chromatography was applied by using 100–200 mesh silica gel. For thin layer chromatography, pre-coated aluminum sheets (silica gel 60F-254, E. Merck) were used. The compounds were visualization with UV-light (254 and 366 nm) or I2 stain and also by spraying with the ceric sulfate reagent.
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3

Characterization of Organic Compounds

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The measurement of optical rotations was performed on a polarimeter (JASCO DIP 360). The IR spectra were obtained through ATR-Tensor 37 spectrophotometer by Bruker. To record the ESI mass spectra the QSTAR XL mass spectrometer by Applied Biosystems was used with a capillary voltage set from 5–5.5 kV. The NMR spectra (1H and 13C) were recorded on Bruker spectrometer operating at 600 MHz (150 MHz for 13C). The δ values on the chemical shift scale are reported in ppm, while the J values of the coupling constants are given in Hz. Purification of minor compounds was performed by recycling HPLC by using a 7:3 EtOAc/n-hexane solvent system in a silica gel column with a flow rate of 4 mL/min. Pre-coated aluminium silica gel sheets (60 F-254, Merck) were used for TLC (thin layer chromatography), which were visualized under UV light at 254 and 366 nm. Ceric sulphate and ninhydrin reagent were used for spraying the TLC followed by heating.
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4

Spectroscopic Characterization of Compound 1

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The purified compound 1 was subjected to spectroscopic analyses (UV, IR, 1H-NMR, 13C-NMR, ESI, and MS/MS studies) for identification and characterization. The optical measurements were conducted by using a polarimeter (JASCO DIP360, Jasco Co., Tokyo, Japan). A Bruker ATR-Tensor 37 spectrophotometer (Bruker, Ettlingen, Germany) was used to record the IR spectra. To obtain the ESI mass spectra, a QSTAR mass spectrometer (Applied Biosystems, Foster, CA, USA) with capillary voltage of 5–5.5 kV was used. The NMR spectra (1H and 13C) were obtained by using a Bruker NMR spectrometer (Burker, Fallanden, Switzerland) operated at 600 MHz and 150 MHz, respectively.
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5

Comprehensive NMR and Mass Spectroscopic Analysis

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The 1H- and 13C NMR spectra were measured on 600 MHz and 150 MHz NMR spectrometer (BRUKER, Zürich, Switzerland) using the solvent peaks (CDCl3, δH: 7.26; δC: 77.0), (CD3OD, δH: 4.87; δC: 48.5) as internal references. Data were determined in the following order: chemical shift (δ) in ppm; multiplicities are indicated s = singlet, d = doublet, t = triplet, dd = doublet of doublet, m = multiplet; coupling constants (J) are in hertz (Hz). High-resolution electrospray ionization mass spectrometry (HR-ESI-MS) spectra were recorded on Agilent 6530 LC Q-TOF (country of origin USA/EU, made in Singapore). Column chromatography was carried out by using silica gel of the selected particle size of 100–200 mesh. For TLC, pre-coated aluminum sheets (TLC silica gel 60 F254, Merck, Darmstadt, Germany) were used. Infrared (IR) spectra were recorded on a Bruker ATR-Tensor 37 spectrophotometer, Bruker (Ettlingen, Baden-Württemberg, Germany) with wave numbers (ν) in cm−1. Visualization was accomplished with UV light (254 and 366 nm) or I2 stain and by spraying with the ceric sulfate (Ce(SO4)2) reagent.
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