Volatile compounds were collected with SPME. A StableFlex 50/30 µm Divinylbenzene/Carboxen/Polydimethylsiloxane fiber (SUPELCO, Bellefonte, PA, USA) was exposed to the vial headspace for 20 min at 40 °C. After collection, analytes were desorbed into a GC-EI-MS system (GCMS-QP2010 SE, Shimadzu, Kyoto, Japan). Desorption was conducted in the splitless mode at 250 °C for 4.5 min. Volatile compounds were separated on a DB-WAX-UI column (60 m, 0.25 mm i.d., 0.25 μm film thickness; Agilent Technologies, CA, USA). For the analysis of standard decomposition, the initial temperature was set at 30 °C, held for 10 min, ramped to 250 °C at 5 °C/min and held for 5 min. In the analysis of volatile compounds from edible oils, the initial temperature was set at 30 °C, held for 10 min, ramped to 250 °C at 50 °C/min and held for 15 min. Helium was used as the carrier gas with a constant linear velocity of 25 cm/sec. EI-MS spectra were obtained in the range of m/z 41–500 at the ion source temperature of 250 °C. Compounds were identified by spectral comparison to the NIST 17 mass spectral library using the GCMS solution software (ver. 4.50).
Db wax ui column
Manufactured by Agilent Technologies
Sourced in United States, Japan
The DB-WAX UI column is a gas chromatography (GC) column designed for the separation and analysis of a wide range of polar compounds. It features a proprietary stationary phase that provides excellent peak shape and resolution for a variety of applications.
Automatically generated - may contain errors
Lab products found in correlation
Gcms qp2010 se, by Shimadzu (1 mentions)
Masshunter quantitative analysis program, by Agilent Technologies (1 mentions)
6890 series gc system, by Agilent Technologies (1 mentions)
7683 series injector, by Agilent Technologies (1 mentions)
Hs 20, by Shimadzu (1 mentions)
Uv 1800 uv vis spectrophotometer, by Shimadzu (1 mentions)
Gc 2010, by Shimadzu (1 mentions)
Dvb carboxen pdms stableflex fiber, by Merck Group (1 mentions)
7890b system, by Agilent Technologies (1 mentions)
8 protocols using db wax ui column
1
Volatile Compound Analysis in Edible Oils
2
Gas Chromatography Analysis of Volatile Organics
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The prepared samples were analyzed by GC (GC; YL6100 Gas Chromatography, Youngin Chromass Co.). The columns used in the experiments were DB-WAX UI (Column; Agilent Technologies), and the carrier gas was N2. The detector type was a flame ionization detector (FID). The temperatures of the inlet and detector were 150 °C and 300 °C, respectively, and the oven temperature was set at 100 °C. Measurements were obtained in isothermal mode.
Fig 2a shows the GC peak from toluene, and Fig 2b shows the GC peaks of the mixed solution of toluene and ethanol. We confirmed that GC peak time was constant and distinguishable for each VOC and ethanol.
3
Quantitative Liver Fatty Acid Profiling
4
Derivatization of enzymatic samples using PFBBr
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The formate was derivatized by modifying a previous method [54 (link)] using 2,3,4,5,6-pentafluoro-benzylbromide(PFBBr). A total of 100 µL of enzyme-free samples were mixed with 50 µL of a 325 mM phosphate buffer, pH 8.5. To this, 365 µL of 100 mM PFBBr solution prepared in acetone was added. The solution was vortexed for 1 min and heated at 60 °C for 20 min. After cooling down to room temperature, 500 µL of n-hexane was added and vortexed for 1 min. Phases were separated by centrifugation at 13,000 rpm for 1 min and the upper organic phase was carefully pipetted into a 2 mL insert containing GC vials. The samples were analyzed by using GC-MS QP2010 SE (Schimadzu) modified for headspace samples. Sample volumes of 1 µL were used in the DB-WAX UI column (30 m × 0.32 mm × 0.25 µm, Agilent).
5
GC-MS Analysis of Organic Compounds
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Gas chromatography-mass spectrometry (GC-MS) analyses were performed using a 6890 Series GC system (Agilent). 0.5 µL of sample was injected through a 7683 Series Injector (Agilent) with a split ratio of 30 and the inlet set at 250°C. Elution was by helium N60 at 0.7 mL/min through a DB-Wax UI column (Agilent) with dimensions 30.0 m × 250 μm x 0.25 µm. The oven temperature was maintained at 50°C for 4 min, then increased up to 95°C at 5°C/min, and finally up to 180°C at 70°C/min with a hold of 5 min. Eluted compounds were detected by a quadrupole 5973 Mass Selective Detector (Hewlett Packard) operating over the scanning range 50–650 m/z, with electron-impact ionization at 70 eV, the transfer line at 250°C, and the source at 230°C. Identification of compounds was based on pure compound injection and mass spectra comparison. The peak areas of each compound were determined from the total ion chromatogram.
6
Microbial Growth Monitoring and Methanol Quantification
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Microbial growth was tracked by measuring absorption at OD600 measured in a UV-1800 UV–Vis spectrophotometer (Shimadzu, Japan). Methanol concentration of the media was measured using a GC-2010 (Shimadzu, Japan) equipped with a DB-WAX UI column (Agilent, Santa Clara, CA) with N2 as carrier gas (at a constant pressure of 100 kPa) and using a flame ionization detector (FID) (at a temperature of 250 °C). Samples were injected using a headspace auto sampler HS-20 (Shimadzu, Japan) with an oven set to 50 °C.
7
Headspace SPME-GC-MS Analysis of Longan Wine Volatiles
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The volatile compounds were analyzed using the headspace (HS) solid‐phase microextraction (SPME) method with a 30/50 µm DVB/Carboxen™/PDMS StableFlex™ fiber (Supelco: 57348‐U, Sigma‐Aldrich) coupled with a gas chromatography (GC) mass spectrometer (MS; HS‐SPME‐GC‐MS). Ten milliliters of longan wine was placed in a 20 ml headspace vial, and then 100 ppm octanol was added into the bottle as the internal standard, to which was added 3.5 g of sodium chloride. The program consisted of swirling the vial at 250 rpm for 10 min at 40°C, inserting the fiber to adsorb at 40°C for 10 min at 250 rpm, and then transferring the fiber for desorption at 230°C for 3 min.
GC‐MS analysis was performed with an Agilent 7890B, equipped with a DB‐Wax UI column (60 m × 0.25 mm coated with 0.25 µm film thickness). The operation conditions were according to Liu et al. (2018 ).
The odor activity values were calculated by dividing the known thresholds.
GC‐MS analysis was performed with an Agilent 7890B, equipped with a DB‐Wax UI column (60 m × 0.25 mm coated with 0.25 µm film thickness). The operation conditions were according to Liu et al. (
The odor activity values were calculated by dividing the known thresholds.
8
Volatile Analysis by HS-SPME-GC-MS
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The volatiles were analyzed by the headspace (HS) solid-phase microextraction (SPME) method with 30/ 50 µm DVB/Carboxen TM /PDMS StableFlex TM fiber (Supelco: 57348-U, Sigma Aldrich, Spain) coupled with GC-MS. GC-MS analysis was performed with an Agilent 7890B system, equipped with a DB-Wax UI column (60 m × 0.25 mm coated with 0.25 µm film thickness). The operation conditions were according to Liu et al. [11] . The program consisted of swirling the vial at 250 rpm for 10 min at 40℃, inserting the fiber to adsorb at 40℃ for 10 min at 250 rpm, and then transferring the fiber for desorption at 230℃ for 3 min.
The odor activity values (OAVs) were calculated as the ratio between the average of the analytical concentrations and the odor thresholds.
The odor activity values (OAVs) were calculated as the ratio between the average of the analytical concentrations and the odor thresholds.
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