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Rat anti elav antibody

Rat anti-Elav antibody is a laboratory reagent used to detect the Elav protein in various biological samples. The Elav protein is a key regulator of neuronal development and function, and the antibody can be used to identify and study Elav-expressing cells.

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2 protocols using rat anti elav antibody

1

Immunostaining of Drosophila Brains

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Fly brains were dissected from 3-to-5-day-old adult females and stained exactly as in Wu and Luo (2006) (link). The following primary antibodies were used: mouse anti-HA antibody (HA.11, Covance, BioLegend: 901514) and rabbit anti-GFP antibody (Abcam: ab290) were used at 1:500, and rat anti-Elav antibody (Developmental Studies Hybridoma Bank, Iowa City, IA, deposited by G. M. Ruben: 7E8A10) was used at 1:25. Cross absorbed secondary antibodies used were: goat anti-mouse IgG Alexa Fluor Plus 555 (Invitrogen: A32727), goat anti-rabbit IgG Alexa Fluor 488 (Invitrogen: A11034), and goat anti-rat IgG Alexa Fluor 647 (Invitrogen: A21247). Brains were imaged on an Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope with a 20X objective.
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2

Immunostaining of Drosophila Brains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly brains were dissected from 3-to-5-day-old adult females and stained exactly as in Wu and Luo (2006) (link). The following primary antibodies were used: mouse anti-HA antibody (HA.11, Covance, BioLegend: 901514) and rabbit anti-GFP antibody (Abcam: ab290) were used at 1:500, and rat anti-Elav antibody (Developmental Studies Hybridoma Bank, Iowa City, IA, deposited by G. M. Ruben: 7E8A10) was used at 1:25. Cross absorbed secondary antibodies used were: goat anti-mouse IgG Alexa Fluor Plus 555 (Invitrogen: A32727), goat anti-rabbit IgG Alexa Fluor 488 (Invitrogen: A11034), and goat anti-rat IgG Alexa Fluor 647 (Invitrogen: A21247). Brains were imaged on an Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope with a 20X objective.
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