The largest database of trusted experimental protocols

2 protocols using rabbit anti pegfr tyr1068

1

Comprehensive Immunoblot Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse anti-HA HA.11 clone 16B12 (#MMS-101P, Covance, 1:1000). Goat anti-BCA2 (#SAB2500854, Sigma Aldrich, 1:500). Rabbit anti-EGFR (#HPA018530, Sigma, 1:1000). Mouse anti-Transferrin Receptor (#13-6800, Life Technologies, 1:500). Mouse anti-c-Met (#3127, Cell Signalling, 1:500). Rabbit anti-pEGFR Tyr1068 (#3777, Cell Signalling, 1,000). Rabbit anti-AKT (#9272, Cell Signalling, 1:1,000). Rabbit anti-pAKT Ser473 (#9271, Cell Signalling, 1:500). Rabbit anti-ERK 1/2 (#9102, Cell Signalling, 1:500). Rabbit anti-pERK 1/2 Thr202/Tyr204 (#9101, Cell Signalling, 1:500). Mouse HRP conjugated anti-β-tubulin (#ab21058, Abcam, 1:50,000). All secondary antibodies for Western blotting were from Pierce and were used at a dilution of 1:2000.
+ Open protocol
+ Expand
2

Investigating HSP70, Cx43, and EGFR Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Low endotoxin rhHSP70 and rhHSP27 were purchased from Enzo Life Sciences (Villeurbanne, Fr) and rabbit anti-HSP70 from ABR (AffinityBioReagent, ThermoFisher, Fr). Mouse anti-Hsc70, rabbit anti-phospho Cx43 (Ser368, Ser255, Ser262 and Tyr265) and probenecid were from Santa Cruz Biotech. Neutralizing anti-TLR4 and mouse anti-EGFR (ErB1) were from Abcam (Cambridge, UK). Mouse anti-TLR4, polymyxin B and gefitinib were from InvivoGen (Toulouse, Fr). Rabbit anti-P-EGFR (Tyr-1068) and antiphospho- and total Akt (Ser473) were from Cell Signaling (Danvers, USA) and mouse anti-Cx43 from Invitrogen. ZO-1 antibody was from Zymed (Invitrogen). DiL-C18, thapsigargin and fura-2/AM were from Molecular Probes. VER155008 and Gap26 (VCYDKSFPISHVR) from Tocris (McKinley, USA). Other chemicals were from Sigma-Aldrich.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!