Immunohistochemical detection kit

Reagents and instruments used in the study are as follows: DSS (MP Biomedicals, UK); moxa sticks for animal experiments (4 mm in diameter and 12 cm in length, Nanyang Hanyi Moxa Co. Ltd., China); ubiquitination inhibitor MG132 (Sigma, USA); NLRP3, IL-1β and Ub primary antibodies (Abcam, UK); RNF183 primary antibody (Novus, USA); IκB α and NF-κB p65 primary antibodies (CST, USA); fluorescent secondary antibodies (Alexa Fluor 647 and Alexa Fluor 488, Abcam, UK); anti-fluorescence quenching sealing liquid (containing DAPI, Beyotime Biotechnology Co. Ltd., China); immunoprecipitation (IP) kit (Thermo Fisher, USA); immunohistochemical detection kit (Dako, Denmark); immunohistochemical detection instruments for dehydration, paraffin-embedding, baking, and spreading (Leica, Germany); fluorescence microscope (Olympus, Japan); Western blotting (WB) instruments for electrophoresis, wet transfer, and gel imaging system (Bio-Rad, USA); and real-time fluorescent quantitative PCR equipment (Roche, Switzerland).

Mouse anti-human iNOS monoclonal antibody and mouse anti-human p53 monoclonal antibody DO-7 were purchased from NeoMarkers (USA). Immunohistochemical detection kit was purchased from Dako (Denmark). Anti-rabbit-IgG-HRP and anti-mouse-IgG-HRP were purchased from Cell Signaling Technology (CST, USA).