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Bradykinin rppgfspfr

Manufactured by Merck Group
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Bradykinin (RPPGFSPFR) is a laboratory reagent produced by Merck Group. It is a peptide composed of nine amino acids that plays a role in various physiological processes. This product is intended for research and scientific applications, and its core function is to facilitate the study of the biological activities and mechanisms associated with bradykinin.

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Lab products found in correlation

Cyano 4 hydroxy cinnamic acid chca, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Toluene, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Drvyihpfhl, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions) α cyano 4 hydroxycinnamic acid chca, by Merck Group (1 mentions) Ammonium hydroxide nh4oh, by Thermo Fisher Scientific (1 mentions) Glacial acetic acid, by Thermo Fisher Scientific (1 mentions) Tetrahydrofuran thf, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) Potassium phosphate dibasic, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Tris hydrochloride, by Merck Group (1 mentions) Dimethyl sulfoxide d6, by Merck Group (1 mentions) Optima lc ms grade, by Thermo Fisher Scientific (1 mentions) Tris 2 carboxyethyl phosphine hydrochloride tcep, by Merck Group (1 mentions) Potassium phosphate monobasic, by Merck Group (1 mentions) Milli q advantage a10 system, by Merck Group (1 mentions)

3 protocols using bradykinin rppgfspfr

1

Peptide Synthesis and Characterization

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The peptide bradykinin (RPPGFSPFR) was purchased from the Sigma-Aldrich. TMR-bradykinin (TAMRA dye conjugated at the N-terminal of the peptide) was obtained from Biopeptek Inc. The following peptides were purchased from the Bachem: kinetensin (IARRHPYFL), malantide (RTKRSGSVYEPLKI), amyloid fragment 1–11 (DAEFRHDSGYE), preproenkephalin (SSEVAGEGDGDSMGHEDLY). Trifluoroacetic acid (TFA), and cyano-4-hydroxy-cinnamic acid (CHCA) were purchased from Sigma-Aldrich.
Zhao B., Serrano M.A., Gao J., Zhuang J., Vachet R.W, & Thayumanavan S. (2017). Self-assembly of random co-polymers for selective binding and detection of peptides. Polymer chemistry, 9(9), 1066-1071.
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2

Peptide Synthesis and Characterization

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The peptides bradykinin (RPPGFSPFR) and angiotensin I (DRVYIHPFHL) were purchased from Sigma-Aldrich. TAMRA-bradykinin (TAMRA dye conjugated at the N-terminus of the peptide) was obtained from Biopeptek Inc. The following peptides were purchased from the American Peptide Company: kinetensin (IARRHPYFL), malantide (RTKRSGSVYEPLKI), β-amyloid fragment 10-20 (DAEFRHDSGYE). Trifluoroacetic acid (TFA), MOPS buffer and α-cyano-4-hydroxy-cinnamic acid (CHCA) were purchased from Sigma-Aldrich. Tris(hydroxymethyl)-aminomethane (Tris buffer), acetonitrile, formic acid, hydrochloric acid (HCl), sodium hydroxide, ammonium hydroxide (NH4OH), glacial acetic acid, toluene, tetrahydrofuran (THF) and purified water were obtained from Fisher Scientific. THF was distilled over Na/Ph2CO before use.
Serrano M.A., Zhao B., He H., Thayumanavan S, & Vachet R.W. (2018). Molecular Features Influencing the Release of Peptides from Amphiphilic Polymeric Reverse Micelles. Langmuir : the ACS journal of surfaces and colloids, 34(15), 4595-4602.
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3

Peptide Mixture Preparation and Analysis

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Chemicals were purchased as
follows: potassium phosphate dibasic (99.9%), potassium phosphate
monobasic (99.9%), tris hydrochloride, tris(2-carboxyethyl)phosphine
hydrochloride (TCEP), and dimethyl sulfoxide-d6 (99.96%) from Sigma;
dimethyl sulfoxide from Fisher Bioreagents; deuterium oxide (99.9%
D) from Goss Scientific; and water (H2O), acetonitrile
(ACN), and formic acid (99.5%) Optima LC/MS Grade were from Fisher
Scientific. All other ultrapure water used was purified on a Milli-Q Advantage A10 system (Merck). Five peptides were used
to prepare the peptide mixture, including bradykinin (RPPGFSPFR) obtained
from Sigma, leucine enkephalin (YGGFL) from Waters, CN-AFP (DTASDAAAAAALTAANAAAAAEKTAADAAAAAAATAA)
from Peptide Synthetics, and cTPRH1 (AEAWYNLGNAYYK) and cTPRS (AEAKQNLGNAKQK)
synthesized in-house on a Biotage parallel synthesizer (SYRO II) on
wang resin with purification by reverse phase against a C8 column
(Polaris). GlyPb from rabbit muscle was purchased from Sigma.
Kish M., Smith V., Lethbridge N., Cole L., Bond N.J, & Phillips J.J. (2023). Online Fully Automated System for Hydrogen/Deuterium-Exchange Mass Spectrometry with Millisecond Time Resolution. Analytical Chemistry, 95(11), 5000-5008.
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