Renilla luciferase prl

Manufactured by Promega

The Renilla-luciferase (pRL) is a reporter gene construct that expresses the Renilla luciferase protein. Renilla luciferase is an enzyme that catalyzes the oxidation of Renilla luciferin, resulting in the emission of light. This reporter gene is commonly used to monitor gene expression and as a control for normalization in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Dual glo luciferase system, by Promega (1 mentions) Longamp taq polymerase, by New England Biolabs (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Pgl3 promoter vector, by Promega (1 mentions) Dual luciferase reporter kit, by Promega (1 mentions) Pgl3 basic vector, by Promega (1 mentions)

Close spelling variants of this product

PRL-CMV Renilla luciferase reporter PRL-null vector expressing renilla luciferase PRL-SV40 Renilla luciferase control reporter vector PRL-CMV renilla luciferase reporter plasmid PRL-TK Renilla luciferase control vector PRL-TK Renilla luciferase PRL-TK constitutively expressing Renilla luciferase PRL-SV40 encoding Renilla luciferase (rLuc) PRL-TK Renilla luciferase plasmid PRL-CMV Renilla luciferase control reporter vector

2 protocols using renilla luciferase prl

Luciferase Reporter Gene Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luciferase-based reporter gene assays were performed in cells using the Dual-Glo luciferase system (Promega). Transfections included 200 ng of renilla-luciferase (pRL) (Promega) as an internal control in addition to 1.8 μg of firefly-based luciferase plasmid. 48 h post-transfection, cells were lysed and analyzed for luciferase activity using a luminometer (BMG Fluostar, ThermoFisher Scientific). Relative reporter activity was calculated and normalized based on renilla-luciferase activity which reflected transfection efficiency. At least three separate experiments were performed, each in triplicate.

Benzina S., Harquail J., Guerrette R., O'Brien P., Jean S., Crapoulet N, & Robichaud G.A. (2016). Breast Cancer Malignant Processes are Regulated by Pax-5 Through the Disruption of FAK Signaling Pathways. Journal of Cancer, 7(14), 2035-2044.

+ Open protocol

+ Expand

Luciferase Assay for Enhancer Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Regions of interest were PCR amplified using LongAMP Taq Polymerase (New England Biolabs) using primers with restriction sites flanking the 5′ ends. These were then cloned into either a pGL3-Basic vector (Promega) or pGL3-promoter vector (Promega) (only for Lif-r-enh). EpiSCs were transfected with both pGL3-derived constructs and Renilla luciferase (pRL) (Promega) using Lipofectamine 2000 (Life Technologies). The dual-luciferase reporter kit (Promega) was used to assay luciferase activation and was measured using the PheraStar Plus plate reader (BMG Technologies). All results were normalized to Renilla luciferase to account for differences in cell number and transfection efficiencies.

Onishi K., Tonge P.D., Nagy A, & Zandstra P.W. (2014). Local BMP-SMAD1 Signaling Increases LIF Receptor-Dependent STAT3 Responsiveness and Primed-to-Naive Mouse Pluripotent Stem Cell Conversion Frequency. Stem Cell Reports, 3(1), 156-168.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!