WT HeLa cells (ATCC: CCL-2, RRID:CVCL_0030) were cultured in Dulbecco’s Modified Eagle Media (DMEM) containing 100 U/ml Penicillin, 0.1 mg/ml Streptomycin, 2 mM L-Glutamine (Cat# AT066; HiMedia Laboratories/Cat# 11965092; Thermo Fisher Scientific) and supplemented with 10% fetal bovine serum. DHC-GFP HeLa cells (Poser et al., 2008 (link)) were cultured in DMEM containing 100 U/ml Penicillin, 0.1 mg/ml Streptomycin, 2 mM L-Glutamine, and 400 µg/ml Geneticin (HiMedia Laboratories) and supplemented with 10% fetal bovine serum (Cat# F7524; Sigma-Aldrich/Moregate Biotech fetal bovine serum—Sterile Filtered). Cells were grown in an incubator at 37°C under 5% CO2.
Geneticin
Manufactured by HiMedia
Sourced in India
Geneticin is a broad-spectrum antibiotic commonly used in molecular biology and cell culture applications. It functions as a selective agent for the identification and isolation of genetically modified cells that have been transfected with a gene conferring resistance to Geneticin.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by HiMedia (1 mentions)
L glutamine, by HiMedia (1 mentions)
Streptomycin, by HiMedia (1 mentions)
Calcium chloride dihydrate, by HiMedia (1 mentions)
Orthophosphoric acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Kanamycin, by HiMedia (1 mentions)
2 protocols using geneticin
1
Culturing WT and DHC-GFP HeLa Cells
2
Molecular Cloning with Chemical Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Orthophosphoric acid, dimethyl sulfoxide, acetyl acetone and ammonium hydroxide were procured from Merck India. Calcium chloride di-hydrate, kanamycin and geneticin were obtained from Himedia, India. kanamycin and geneticin resistance gene containing plasmids and agarose were purchased from Genei, India.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!