The groups were as follows: Control, CORM + GSNO, CCOD-GNs-4-MAP, Surgical delay, and CN-Patch (n = 6). Rats were anesthetized by isoflurane inhalation and the hairs on the back areas were shaved. Anesthetized rats were placed in a prone position and injected with IR-780@BSA probe (6 mM, 3 ml/kg) via tail vein51 (link),52 (link). All NIR-II images were obtained within 3 min after injection of the probe, using a two-dimensional InGaAs camera (Princeton Instruments, NIRvana-640). Images were collected with an 1100 nm long pass filter and 40 ms exposure time, under an 808 nm laser excitation (65 mW/cm2). To maximize image quality, each flap image was pieced together from three segments: anterior, mid, and posterior parts using ImageJ software (ImageJ v.1.52a) and Photoshop (Adobe, CC2018).
Imagej v 1.52a
Manufactured by Adobe
ImageJ v.1.52a is an open-source image processing program designed for scientific use. It can display, edit, analyze, process, save, and print 8-bit, 16-bit, and 32-bit images. ImageJ supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection, and median filtering.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using imagej v 1.52a
1
Near-Infrared Imaging of Surgical Flaps
2
Chromosome Imaging and Quantification
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Chromosome spreads were imaged by confocal microscopy (Andor Dragonfly spinning disc confocal microscope driven by Fusion Software) and Imaris software was used to process the images. Histological samples were taken by fluorescence microscope (bx52, Olympus), Photoshop (Adobe), and ImageJ (v.1.52a) were used for image processing to count foci and quantify fluorescence.
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