Nadp nadph glo assays kit

Manufactured by Promega

Sourced in United States

The NADP/NADPH-Glo™ Assays kit is a bioluminescent assay designed to measure the levels of NADP and NADPH in biological samples. The kit utilizes a coupled enzymatic reaction that generates a stable luminescent signal proportional to the amount of NADP or NADPH present.

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Lab products found in correlation

Synergy h1 plate, by Agilent Technologies (1 mentions) Nad nadh glo, by Promega (1 mentions) Nadph standard, by Abcam (1 mentions)

Close spelling variants of this product

NADP/NADPH-Glo Assay Kit NADP/NADPH-Glo Assay NADP/NADPH-GloTM assay NADPH/NADP+ Assay kit NADPH-Glo assay kit NADP/NADPH-Glo NADPH-Glo assay NADPH-Glo kit NADPH

2 protocols using nadp nadph glo assays kit

Quantification of NAD(P)(H) Levels

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NAD⁺, NADP⁺, NADH, and NADPH levels, as well as NAD+/NADH and NADP⁺/NADPH ratios were examined using the NAD/NADH-Glo™ or NADP/NADPH-Glo™ Assays kit (Promega, USA) according to the manufacturer’s protocols. Briefly, 10.000 cells were plated in a 96 well plate 24 h before treatment. Cells were lysed followed by measuring NAD(P)⁺ or NAD(P)H luminescence signal separately by using BioTek Synergy H1 plate reader (Biotek, USA). The ratio of NAD+/NADH and NADP+/NADPH were calculated based on the instruction of the kits.

Xiang K., Kalthoff C., Münch C., Jendrossek V, & Matschke J. (2022). Accumulation of oncometabolite D-2-Hydroxyglutarate by SLC25A1 inhibition: A metabolic strategy for induction of HR-ness and radiosensitivity. Cell Death & Disease, 13(7), 641.

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Quantifying NADP+, NADPH, and ATP

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NADP⁺ and NADPH were also quantified using the NADP/NAPDH quantitation colorimetric assays with a NADPH standard (BioVision, Inc, San Francisco, CA) and normalized by protein concentration. The NADP/NADPH ratio was measured by a NADP/NADPH-Glo™ assays kit (Promega, Madison, WI), following the manufacturers’ protocol, and the relative NADPH/NADP⁺ ratio is normalized by that of control cells (or treatment control) under matrix-attached condition. ATP levels were detected by an ATP colorimetric assays kit (Abcam) and normalized by protein concentration, and the relative ATP levels is normalized by that of control cells (or treatment control) under matrix-attached condition.

Zhang Y., Xu Y., Lu W., Ghergurovich J.M., Guo L., Blair I.A., Rabinowitz J.D, & Yang X. (2020). Upregulation of antioxidant capacity and nucleotide precursor availability suffices for oncogenic transformation. Cell metabolism, 33(1), 94-109.e8.

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