Anti zo 1 antibody

Cell lysate combinations of RIPA with PIC2 and PPI were used to form the lysis buffer, and a reaction time of 30 min in an ice bottle was allocated. The protein concentration in each cell lysate was then measured using a commercial BCA kit (Thermo Fisher Scientific, Waltham, MA, USA). In the Western blot analysis, an SDS-polyacrylamide gel electrophoresis system was used. An anti-GAPDH antibody (Cell Signaling, Danvers, MA, USA), anti-fibronectin antibody (Abcam, Cambridge, England, UK), anti-vimentin antibody (cell signaling), anti-E-cadherin antibody (BD Biosciences, Franklin Lakes, NJ, USA), anti-ZO-1 antibody (BD Biosciences), anti-PCNA antibody (Cell Signaling), anti-protein kinase B (AKT) antibody (Cell Signaling), anti-phospho-AKT (P-AKT) antibody (Cell Signaling), anti-α7-nAChR antibody (Abcam), and anti-SNCG antibody (Cell Signaling) were used for probing.

Anti-HIF-1α antibody (cat. no. 610959) was obtained from BD (San Jose, CA, USA); anti-ZO-1 antibody (cat. no. bs-1329R) from Bioss Antibodies (Woburn, MA, USA); anti-occludin antibody (cat. no. NBP1–77037) from Novus Biologicals (Littleton, CO, USA); anti-claudin-1 antibody (cat. no. 13255) and anti-β-Actin antibody (cat. no. 58169) from Cell Signaling Technology (Beverly, MA, USA). Gelatin was purchased from Acros Organics (Morris Plains, NY, USA). CAY10585 was obtained from BioVision (Milpitas, CA, USA). The mirVana® miRNA mimic (has-miR-29b-3p, cat. no. MC10103), Negative Control #1, and Lipofectamine™ RNAiMAX Transfection Reagent were purchased from Thermo Fisher Scientific (NY, USA). All other chemicals were purchased from Fisher (Pittsburgh, PA, USA) unless otherwise indicated. All chemicals used were of analytic grade.