Tak 165

EBM-2 Basal Medium and EGM-2 SingleQuot Kit supplement/growth factors were purchased from Lonza Walkersville, Inc. (Walkersville, MD), and EGM Cell Growth Medium-2 was prepared according the manufacturer’s instructions. NRG-1 (ECD, 377-HB) and EGF (236-EG) were purchased from Bio-Techne/R&D Systems. The recombinant human glial growth factor 2 (GGF2; neuregulin-1beta3; USAN - cimaglermin alfa) was provided by Acorda Therapeutics (Ardsley, NY). Calcein AM and 7-AAD were from ThermoFisher Scientific/Molecular probes. AG-1478 and TAK-165 were obtained from Tocris Bioscience (Bristol, UK). AST-1306 was purchased from SelleckChem (Houston, TX). Collagenase II (345 units per mg, CLS-2) was purchased from Worthington biochemical Corporation (Lakewood, NJ), dispase II (04942078001) was from Roche Life Science (Indianapolis, IN) and DNase I was from Sigma (St. Louis, MO). For experiments involving cell stimulation, final concentrations of dimethyl sulfoxide (Cell Culture grade, Sigma) did not exceed 0.1%.

EBM-2 Basal Medium and EGM-2 SingleQuot Kit supplement/growth factors were purchased from Lonza Walkersville, Inc. (Walkersville, MD), and EGM Cell Growth Medium-2 was prepared according to the manufacturer’s instructions. NRG-1 (ECD, 377-HB) and EGF (236-EG) were purchased from Bio-Techne/R&D Systems. TAK-165 and GI 254023X were obtained from Tocris Bioscience (Bristol, UK) and diluted in dimethyl sulfoxide (Cell Culture grade, Sigma). Collagenase II (345 units per mg, CLS-2) was purchased from Worthington Biochemical Corporation (Lakewood, NJ), dispase II (04942078001) was from Roche Life Science (Indianapolis, IN). D-glucose and DNase I was from Sigma (St. Louis, MO). For experiments involving cell stimulation, final concentrations of dimethyl sulfoxide did not exceed 0.1%.