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Human igg quantitation elisas

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The Human IgG quantitation ELISAs are laboratory assays designed to quantify the levels of human immunoglobulin G (IgG) in samples. These ELISAs provide a quantitative measurement of IgG concentrations, which is useful for various research and diagnostic applications.

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Lab products found in correlation

Nanodrop spectrophotometer, by Thermo Fisher Scientific (4 mentions) Fectopro, by Polyplus Transfection (2 mentions) Ammag protein a magnetic beads, by GenScript (1 mentions) Ammag protein a beads, by GenScript (1 mentions)

2 protocols using human igg quantitation elisas

1

Recombinant Monoclonal Antibody Production

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Heavy chain and light chain variable sequences were codon optimized and cloned into in-house vectors, containing human IgG1 constant region or kappa or lambda constant regions, respectively. Expi293F cells were transfected with heavy chain and light chain plasmids using FectoPro (Polyplus transfection). Media was harvested after seven days, and mAbs were purified with AmMag Protein A magnetic beads (Genscript). Antibody concentrations were measured with a nanodrop spectrophotometer (Thermo Fisher Scientific) and human IgG quantitation ELISAs (Bethyl Laboratories).
Brewer R.C., Ramadoss N.S., Lahey L.J., Jahanbani S., Robinson W.H, & Lanz T.V. (2021). BNT162b2 vaccine induces divergent B cell responses to SARS-CoV-2 S1 and S2. Nature immunology, 23(1), 33-39.
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2

Single-cell Antibody Profiling Post-BNT162b2

Cited 1 time
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All heavy and light-chain (HC and LC) sequences were obtained by single-cell repertoire sequencing from individuals on days 7, 21, and 28 post initial BNT162b2 vaccination (second immunization on day 21)7 (link). Codon-optimized HC and LC variable sequences were cloned into in-house vectors, containing human IgGI and κ or λ constant regions, respectively. HC and LC plasmids were transfected into Expi293F cells using FectoPro (Polyplus transfection). Cell supernatants were collected after 7 days and purified with AmMag Protein A beads (Genscript). mAb concentrations were measured using a nanodrop spectrophotometer (Thermo Fisher Scientific) and human IgG quantitation ELISAs (Bethyl Laboratories).
Lanz T.V., Brewer R.C., Jahanbani S, & Robinson W.H. (2022). Limited Neutralization of Omicron by Antibodies from the BNT162b2 Vaccination against SARS-CoV-2. Research Square.
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