Five “cluster of differentiation” markers previously identified in our laboratory were used to assess the ability of WT SRCAP and FHS MUT SRCAP lines to make neural crest cells (Prescott et al. 2015 (link)). These markers were CD10 (MME), CD99, CD105 (ENG), CD266 (TNFRSF12A) and CD271 (NGFR, p75) (Miltenyi Biotec). Cells were stained with surface marker antibodies in FACS buffer (5% FBS, 5mM EDTA, 0.1% sodium azide in PBS) and surface expression of each marker was determined using flow cytometry (AriaII SORP and BD Fortessa). All antibodies used (5μL each per sample) for surface marker analysis: CD10-APC, CD24 PerCP-Vio700, CD99-APC-Vio770, CD105-VioBlue, CD266-PE, CD271-PE-Vio770 (Miltenyi).
Cd24 percp vio700
Manufactured by Miltenyi Biotec
Sourced in Germany
The CD24-PerCP-Vio700 is a fluorescently-labeled antibody that binds to the CD24 antigen. CD24 is a cell surface glycoprotein expressed on various cell types. The PerCP-Vio700 fluorescent label allows for the detection and analysis of CD24-positive cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Facssuite, by BD (2 mentions)
Anti igd vioblue, by Miltenyi Biotec (2 mentions)
Cd21 apc vio770, by Miltenyi Biotec (2 mentions)
Anti igm pe, by Miltenyi Biotec (2 mentions)
Cd38 fitc, by Miltenyi Biotec (2 mentions)
Cd19 viogreen, by Miltenyi Biotec (2 mentions)
Facslyric flow cytometer, by BD (2 mentions)
Cd27 apc, by Miltenyi Biotec (2 mentions)
Cd86 pe vio770, by Miltenyi Biotec (2 mentions)
Cd105 vioblue, by Miltenyi Biotec (1 mentions)
Cd105, by Miltenyi Biotec (1 mentions)
3 protocols using cd24 percp vio700
1
Neural Crest Cell Characterization
2
Immunophenotyping of Lymphocytes and B-cells
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Blood samples from the baseline visit were processed within 4 hours for analysis by flow cytometry. For lymphocyte phenotyping, ethylenediaminetetraacetic acid whole blood was stained for CD3, CD4, CD8, CD45, CD16, CD56, and CD19, as previously described (30 (link)). For B-cell phenotyping, PBMCs were isolated from lithium heparin whole blood by Ficoll gradient density centrifugation. One million PBMCs were incubated with the following antibodies: CD19-VioGreen, anti-IgD-VioBlue, CD24-PerCP-Vio700, CD38-FITC, CD27-APC, CD86-PE-Vio770, CD21-APC-Vio770, and anti-IgM-PE (Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were measured using a FACSLyric flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). Data were analyzed using the FACSSuite (BD Biosciences). The gating strategy is shown in Supplemental Figure 1
3
Comprehensive Immune Cell Profiling
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Blood samples from the baseline visit were processed within 4 h for analysis by flow cytometry. For lymphocyte phenotyping, ethylenediaminetetraacetic acid whole blood was stained for CD3, CD4, CD8, CD45, CD16, CD56, and CD19. For immune cell phenotyping, PBMCs were isolated from lithium heparin whole blood by Ficoll gradient density centrifugation. One million PBMCs were incubated with the following antibodies: CD19-VioGreen, anti-IgD-VioBlue, CD24-PerCP-Vio700, CD38-FITC, CD27-APC, CD86-PE-Vio770, CD21-APC-Vio770, and anti-IgM-PE (Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were measured using a FACSLyric flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). Data were analyzed using FACSSuite (BD Biosciences).
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