A Perkin-Elmer GC-MS system with a gas chromatograph interfaced to a mass spectrometer (GC-MS) and an Elite-1 fused silica capillary column (30 m × 0.25 mm IDX 1 DF, made entirely of dimethylpolysiloxane) were used to conduct the GC-MS analysis of the methanol extract of C. asiatica. The electron ionization device utilized for GC/MS detection had an ionizing energy of 70 eV. With a split ratio of 10:1, a constant flow rate of 1 mL/min, an injection volume of 2 l, and temperatures of 250 °C for the injector and 280 °C for the ion source, helium gas (99.999%) was used as the carrier gas. Further, the chemical constituents identified from the extract were identified by comparing their chromatograms and mass spectral data with those of reference standard compounds from the NIST-09 library.
Elite 1 fused silica capillary column
Manufactured by PerkinElmer
Sourced in United States
The Elite-1 fused silica capillary column is a high-performance chromatographic column designed for a variety of analytical applications. It features a fused silica capillary construction and is suitable for use with gas chromatography systems.
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2 protocols using elite 1 fused silica capillary column
1
GC-MS Analysis of C. asiatica Methanol Extract
2
GC-MS Analysis of Volatile Compounds
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GC-MS analysis was carried out on a GC clarus 500 Perkin Elmer system (USA) comprising a AOC-20i auto sampler and gas chromatograph interfaced to a mass spectrometer instrument employing the following conditions: column Elite-1 fused silica capillary column (30 × 0.25 mm ID × 1μ Mdf, composed of 100% Dimethyl polysiloxane), operating in electron impact mode at 70 eV; Helium gas (99.999%) was used as carrier gas at a constant flow of 1 mL/min and an injection volume of 0.5 μl was employed (split ratio of 10:1) injector temperature 250 °C, ion-source temperature 280 °C. The oven temperature was programmed from 110 °C (isothermal for 2 min), with an increase of 10 °C/min, to 200 °C, then 5 °C/min to 280 °C, ending with a 9 min isothermal at 280 °C. Mass spectra were taken at 70 eV; a scan interval of 0.5 s and fragments from 40 to 450 Da. Total GC running time is 36 min. The relative percentage amount of each component was calculated by comparing its average peak area to the total areas. TurboMass Ver 5.2.0 software was employed to handle mass spectra and chromatograms.18
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