Immunolabeling for matrix metalloproteinase-1 (MMP1), MMP3, MMP7, MMP9 and MMP12 was performed on fixed and paraffin-embedded sections. Briefly, following deparaffinization and rehydration, slides were subjected to antigen retrieval in citrate buffer (pH 6.0) for 20 min in a steamer, rinsed in PBS (0.01 mol/L phosphate buffer containing 0.15 mol/L NaCl, pH7.4), and incubated for 10 min in 3% H2O2 to block endogenous peroxidases. After that, the sections were incubated with anti-MMP1 (1:200; Bioss, China), anti-MMP3 (1:200; Bioss, China), anti-MMP7 (1:200; Bioss, China), anti-MMP9 (1:200; Bioss, China), and anti-MMP12 (1:200; Bioss, China) primary antibodies at 4°C overnight in a wet box, and then with HRP-labeled anti-rabbit secondary antibodies for 30 min, followed by counterstaining with hematoxylin, rehydration and mounting. Staining intensity was quantified by Image-Pro Plus 6.0 (Media Cybernetics) and expressed as mean using the formula “mean density = integrated optical density/area of interest”.
Anti mmp1
Manufactured by Bioss Antibodies
Sourced in China
Anti-MMP1 is a lab equipment product developed by Bioss Antibodies. It is an antibody that specifically binds to the Matrix Metalloproteinase 1 (MMP1) protein. MMP1 is an enzyme involved in the breakdown of extracellular matrix components. The primary function of Anti-MMP1 is to detect and quantify the presence of MMP1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti mmp 9, by Bioss Antibodies (2 mentions)
Anti mmp3, by Bioss Antibodies (2 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
Ecl reagent, by AbFrontier (1 mentions)
Hrp conjugated anti rabbit igg antibody, by GeneTex (1 mentions)
Anti β actin, by Bioss Antibodies (1 mentions)
Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Anti inos, by Bioss Antibodies (1 mentions)
Anti cox 2, by Bioss Antibodies (1 mentions)
Anti p p38, by Bioss Antibodies (1 mentions)
Anti p erk1 2, by Bioss Antibodies (1 mentions)
Sybr premix ex taq 2, by Transgene (1 mentions)
Gapdh, by Abcam (1 mentions)
3 protocols using anti mmp1
1
Immunolocalization of Matrix Metalloproteinases
2
Western Blot Analysis of MMP1 in Breast Cancer
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The total protein was extracted from cell lysates and Western blot analysis was performed as described previously [32 (link)]. Briefly, the breast cancer cells were suspended in RIPA buffer containing 1% Halt protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). The protein (35 μg) was blotted with either anti-MMP1 (1:400, Bioss, Woburn, MA, USA, bs-0424R) or anti-β-actin (1:1000, Bioss, bs-0061R) overnight, followed by incubation with an HRP-conjugated anti-rabbit IgG antibody (1:1000, Genetex, Irvine, CA, USA, GTX213110-01) for 2 h. The bands were visualized using the ECL reagent (Abfrontier, Seoul, Korea).
3
Investigating Inflammatory Pathways in Cells
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IL-1β was obtained from T&L Biotechnology Co., LTD. (Beijing, China). DMEM medium and FBS were obtained from Genetime Biotechnology Co., Ltd. (Shanghai, China). Primescript™ reverse transcription kit and SYBR® Premix Ex Taq™ II were purchased from TransGen Biotech (Beijing, China). Antibodies including anti-MMP-1, anti-MMP-3, anti-MMP-9, anti-ADAMS-4, anti-ADAMS-5, anti-collagen, anti-aggrecan, anti-iNOS, anti-COX-2, anti-p-p38, anti-p-ERK1/2, and anti-p-p65 were all obtained from Bioss Biotechnology Co., Ltd. (Beijing, China). GAPDH was purchased from Abcam Inc. (Cambridge, U.K.). The sequences of primers used in the present study was obtained from Tsingke (Beijing, China). ELISA kits were purchased from MSKBIO Technology Ltd. (Wuhan, China).
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