Electrochemical detection

Manufactured by Eicom

Sourced in United States

Electrochemical detection is a laboratory equipment that measures and analyzes the electrical properties of a sample. It is used to detect and quantify the presence of specific chemical compounds in a solution or sample.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using electrochemical detection

Quantification of Acetylcholine by HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

All dialyzate samples were initially frozen and later unthawed to assay for ACh using high-performance liquid chromatography (HPLC) with electrochemical detection (Eicom, United States). Acetylcholine peaks were quantified by comparison to peak heights of standard solutions (100 nm, 20 nm, and 4 nm standards). Chromatographs obtained every 18 min/sample was analyzed using the software program Envision (provided by Eicom, United States). Minimal detection was 5 femtomoles of ACh.

Reitz N.L., Nunes P.T, & Savage L.M. (2021). Adolescent Binge-Type Ethanol Exposure in Rats Mirrors Age-Related Cognitive Decline by Suppressing Cholinergic Tone and Hippocampal Neurogenesis. Frontiers in Behavioral Neuroscience, 15, 772857.

+ Open protocol

+ Expand

Quantifying Striatal Neurotransmitters by HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) levels in the striatum were measured by HPLC with electrochemical detection. Briefly, after the mice were sacrificed, we rapidly dissected the striatum and the neurotransmitters were extracted in 100 μL of ice-cold perchloric acid via sonication. Homogenates were centrifuged at 13,200 × g for 10 min at 4°C. Supernatants were filtered (pore size: 0.45 μm; Millipore, MA, USA) and then injected directly into the HPLC system. The dopamine, DOPAC, and HVA contents were determined with electrochemical detection (Eicom Corp., Kyoto, Japan). Concentrations of dopamine and its metabolites were expressed as ng/mg tissue weight.

Li R, & Chen J. (2019). Salidroside Protects Dopaminergic Neurons by Enhancing PINK1/Parkin-Mediated Mitophagy. Oxidative Medicine and Cellular Longevity, 2019, 9341018.

+ Open protocol

+ Expand

Acetylcholine Quantification in Dialysate

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dialysate samples were assayed for acetylcholine using HPLC with electrochemical detection (Eicom USA, San Diego, CA). Acetylcholine peaks were quantified by comparison to peak heights of standard solutions and corrected for in vitro recovery of the probe. The system detection limit is reliably 5 femtomole of acetylcholine. Chromatographs obtained every 15 min/sample were analyzed using the software program Envision (provided by Eicom, USA).

Kirshenbaum G.S., Chang C.Y., Bompolaki M., Bradford V.R., Bell J., Kosmidis S., Shansky R.M., Orlandi J., Savage L.M., Harris A.Z., David Leonardo E, & Dranovsky A. (2023). Adult-born neurons maintain hippocampal cholinergic inputs and support working memory during aging. Molecular psychiatry, 28(12).

+ Open protocol

+ Expand

Quantifying Acetylcholine Levels via HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The dialysate samples collected from the HPC and PFC were frozen and later analyzed for ACh using high-performance liquid chromatography (HPLC) with electrochemical detection (Eicom, USA). Sample derived ACh peaks were compared with standard peaks (100 nm, 20 nm, and 4 nm) for the quantification of ACh content in femtomoles. Chromatographs were analyzed using Envision software (Eicom, USA). The minimum detection of the system for ACh analysis was 5 femtomoles.

Kipp B.T., Nunes P.T, & Savage L.M. (2021). Sex Differences in Cholinergic Circuits and Behavioral Disruptions Following Chronic Ethanol Exposure with and without Thiamine Deficiency. Alcoholism, clinical and experimental research.

+ Open protocol

+ Expand

Acetylcholine Quantification via HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dranovsky A., Kirshenbaum G., Chang C.Y., Bompolaki M., Bradford V., Bell J., Kosmidis S., Shansky R., Orlandi J., Savage L., Leonardo E, & Harris A. (2023). Adult-born neurons maintain hippocampal cholinergic inputs and support working memory during aging. Research Square.

+ Open protocol

+ Expand

Acetylcholine Quantification in Brain Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dialysate samples were assayed for ACh using high performance liquid chromatography (HPLC) with electrochemical detection (Eicom USA). ACh peaks were quantified by comparison to peak heights of standard solutions (100 nm, 20 nm, and 5 nm standards). Chromatographs obtained every 18 minute/sample were analyzed using the software program Envision (provided by Eicom, USA). Minimal detection was 5 femtomoles of ACh. Due to a blockage in one hippocampal probe, dialysate was collected from 9 EtOH pre- treated animals. The final n’s for collection in the PFC and hippocampus are noted on Figure 2-C and 2-D.

Fernandez G.M, & Savage L.M. (2017). Adolescent Binge Ethanol Exposure Alters Specific Forebrain Cholinergic Cell Populations and Leads to Selective Functional Deficits in the Prefrontal Cortex. Neuroscience, 361, 129-143.

+ Open protocol

+ Expand

Acetylcholine Detection in Dialysate

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dialysate samples were assayed for ACh using HPLC with electrochemical detection (Eicom USA, San Diego, CA). ACh peaks were quantified by comparison to peak heights of standard solutions and corrected for in vitro recovery of the probe. The system detection limit is reliably 5 femtomole of ACh. Chromatographs obtained every 15 min/sample were analyzed using the software program Envision (provided by Eicom, USA).

Hall J.M, & Savage L.M. (2016). Exercise leads to the re-emergence of the cholinergic/nestin neuronal phenotype within the medial septum/diagonal band and subsequent rescue of both hippocampal ACh efflux and spatial behavior. Experimental neurology, 278, 62-75.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!