Mouse monoclonal anti caspase 3

Lovastatin (Sigma-Aldrich, St. Louis, MO, USA)was converted to an active form as described previously (26 (link)). Briefly, inactive Lovastatin was dissolved in warm (55°C) ethanol, followed by the addition of 0.6 M NaOH and H₂O, and was incubated at room temperature for 30 min. Subsequently, HCl was used to adjust the final solution to pH 8.0, and the Lovastatin solution was stored as a 10 mM stock at −20°C. Rabbit polyclonal anti-SGK3 (1:1,000, ab153981), rabbit monoclonal anti-ATP2B1 (1:1000, ab190355), rabbit monoclonal anti-PTK2B (1:1,000, ab32571), rabbit polyclonal anti-eNOS (1:1,000, ab5589), rabbit monoclonal anti-MAP3K3 (1:2,000, ab40756) and rabbit polyclonal MAP4K3 (1:2,000, ab103481) antibodies were purchased from Abcam (Cambridge, MA, USA). In addition, rabbit polyclonal anti-BCL2 (1:1,000, 2872), rabbit monoclonal anti-BAX (1:1,000, 5023), mouse monoclonal anti-caspase-3 (1:500, 9668), mouse monoclonal anti-AKT(1:2,000, 2920), rabbit monoclonal anti-p-AKT (1:1,000, 4060) and mouse monoclonal β-actin (1:5,000, 3700) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Rabbit polyclonal anti‐ELOVL6 (Millipore Corporation, Billerica, MA, USA), mouse monoclonal anti‐SCD1 (Abcam Tokyo, Japan), rabbit polyclonal anti‐PARP, mouse monoclonal anti‐caspase‐3, rabbit polyclonal anti‐cleaved caspase‐3, rabbit monoclonal anti‐PERK, rabbit monoclonal anti‐phospho‐PERK, and rabbit monoclonal anti‐beta‐actin for an internal standard (Cell Signaling Technology, Beverly, MA, USA) were used. Immunoreactive proteins were detected using an enhanced chemiluminescence system (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and an LAS‐3000 Luminescent Image Analyzer (Fujifilm, Tokyo, Japan).