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Goat anti mouse igg alexa fluor plus 647

Manufactured by Thermo Fisher Scientific
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Goat anti-mouse IgG Alexa Fluor Plus 647 is a secondary antibody conjugated with the Alexa Fluor Plus 647 fluorophore. It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies in various immunoassay applications.

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Lab products found in correlation

Sp8 sted 3x confocal microscope, by Leica (2 mentions) Goat anti chicken igy alexa flour plus 647, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg alexa flour 488, by Abcam (2 mentions) Trueview autofluorescence quenching kit, by Vector Laboratories (2 mentions) Imaris, by Oxford Instruments (2 mentions) Mouse anti ha antibody ha 11 clone 16b12, by BioLegend (1 mentions) Goat anti rabbit igg alexa fluor plus 647, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Alexa Fluor 647 goat anti-mouse IgG Alexa Fluor 647 anti-goat IgG Alexa Fluor 647 goat anti-mouse Alexa Fluor 647 anti-mouse IgG Alexa 647 goat anti-mouse IgG Alexa Fluor Plus 647 IgG Alexa Fluor goat anti-mouse IgG Anti-IgG Alexa Fluor 647 Anti-mouse Alexa Fluor 647 Goat anti-mouse Alexa 647

3 protocols using goat anti mouse igg alexa fluor plus 647

1

Characterization of Protein Tagging Antibodies

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The following commercially available antibodies were used: mouse anti-HA antibody HA.11 (clone 16B12, Biolegend, Cat# 901503, RRID:AB_2565005), rabbit anti-DYKDDDDK (FLAG) tag antibody (clone D6W5B, Cell Signaling Technology Cat# 14793, RRID:AB_2572291), HRP-coupled rabbit anti-E tag antibody (Bethyl Laboratories Cat# A190-133P, RRID:AB_345222), HRP-coupled MonoRab rabbit anti-camelid VHH antibody (clone 96A3F5, GenScript Cat# A01860-200, RRID:AB_2734123), HRP-coupled mouse anti-HA-Tag (clone 6E2, Cell Signaling Technology Cat# 2999, RRID:AB_1264166), goat anti-mouse IgG Alexa Fluor Plus 647 (Thermo Fisher Scientific Cat# A32728, RRID:AB_2633277), goat anti-rabbit IgG Alexa Fluor Plus 647 (Thermo Fisher Scientific Cat# A32733, RRID:AB_2633282).
Koenig P.A., Das H., Liu H., Kümmerer B.M., Gohr F.N., Jenster L.M., Schiffelers L.D., Tesfamariam Y.M., Uchima M., Wuerth J.D., Gatterdam K., Ruetalo N., Christensen M.H., Fandrey C.I., Normann S., Tödtmann J.M., Pritzl S., Hanke L., Boos J., Yuan M., Zhu X., Schmid-Burgk J.L., Kato H., Schindler M., Wilson I.A., Geyer M., Ludwig K.U., Hällberg B.M., Wu N.C, & Schmidt F.I. (2021). Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape. Science (New York, N.y.), 371(6530), eabe6230.
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2

Multi-Fluorescent Immunohistochemistry Protocol

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Specimen slides were prepared as described above. Tissues were incubated overnight at 4°C with primary antibodies. After washing in PBS, specimens were incubated with a mixture of secondary antibodies (goat anti‐chicken IgY Alexa Flour Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32933], goat anti‐rabbit IgG Alexa Flour 488 [Abcam, Cambridge MA; #ab150077] and/or goat anti‐mouse IgG Alexa Fluor Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32728]) for 1–2 h at room temperature. The slides were then rinsed and stained with DAPI using the TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA; #SP‐8400‐15) according to the manufacturer's protocol. Images were captured using a Leica SP8 STED 3X confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). Acquired Z‐stacks and figure images were generated using Imaris (Oxford Instruments, Zurich, CH) and Fiji ImageJ (version 1.0, https://imagej.net/Fiji) software programs.44
Nonnecke E.B., Castillo P.A., Johansson M.E., Hollox E.J., Shen B., Lönnerdal B, & Bevins C.L. (2022). Human intelectin‐2 (ITLN2) is selectively expressed by secretory Paneth cells. The FASEB Journal, 36(3), e22200.
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3

Immunofluorescence Staining of Cell Samples

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Specimen slides were prepared as described above. Tissues were incubated overnight at 4°C with primary antibodies. After washing in PBS, specimens were incubated with a mixture of secondary antibodies (goat anti-chicken IgY Alexa Flour Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32933], goat anti-rabbit IgG Alexa Flour 488 [Abcam, Cambridge MA; #ab150077] and/or goat anti-mouse IgG Alexa Fluor Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32728]) for 1–2 h at room temperature. The slides were then rinsed and stained with DAPI using the TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA; #SP-8400–15) according to the manufacturer’s protocol. Images were captured using a Leica SP8 STED 3X confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). Acquired Z-stacks and figure images were generated using Imaris (Oxford Instruments, Zurich, CH) and Fiji ImageJ (version 1.0, https://imagej.net/Fiji) software programs (44 (link)).
Nonnecke E.B., Castillo P.A., Johansson M.E., Hollox E.J., Shen B., Lönnerdal B, & Bevins C.L. (2022). Human intelectin‐2 (ITLN2) is selectively expressed by secretory Paneth cells. The FASEB Journal, 36(3), e22200.
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